caa a22 4500 465763901 CHVBK 20180323111907.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00914033 doi (NATIONALLICENCE)springer-10.1007/BF00914033 Deglycosylation of neutral and acidic human colonic mucin [Elektronische Daten] [Sambhu Bhattacharyya, John Enriquez Sr., Bridget Manna] Human colonic mucin has been isolated from normal colonic mucosa by a phenol-water extraction procedure and purified by Sepharose 2B column chromatography. The mucin was further purified by cesium bromide density gradient centrifugation. Sodium dodecyl sulfate-polyacrylamide gel (5%) electrophoresis of this material showed high-molecular-weight mucin component(s) at the top of the gel. Chemical analyses of this preparation indicated a typical mucin profile of amino acids and carbohydrates. Ion-exchange chromatography resulted in the separation of two major fractions, one being more acidic than the other. Chemical deglycosylation of the purified preparation at 20°C for 3 1/2 showed loss of sialic acid, fucose, galactose, andN-acetylglucosamine, whereas traces ofN-acetylgalactosamine were still detected. High-pressure liquid chromatography of the deglycosylated material resulted in the purification of a major peptide, P1; with high levels of threonine, serine, and proline, resembling, in most respects, the profile of native mucin. The molecular weight of the peptide was determined to be approximately 97 kDa and serine was the single NH2 terminus. Plenum Publishing Corporation, 1990 Bhattacharyya Sambhu Department of Clinical Investigation, William Beaumont Army Medical Center, 79920-5001, El Paso, Texas, USA aut Enriquez Sr. John Department of Clinical Investigation, William Beaumont Army Medical Center, 79920-5001, El Paso, Texas, USA aut Manna Bridget Department of Clinical Investigation, William Beaumont Army Medical Center, 79920-5001, El Paso, Texas, USA aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/1(1990-02-01), 93-107 0360-3997 14:1<93 1990 14 10753 https://doi.org/10.1007/BF00914033 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914033 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bhattacharyya Sambhu Department of Clinical Investigation, William Beaumont Army Medical Center, 79920-5001, El Paso, Texas, USA aut NATIONALLICENCE 700 1- Enriquez Sr John Department of Clinical Investigation, William Beaumont Army Medical Center, 79920-5001, El Paso, Texas, USA aut NATIONALLICENCE 700 1- Manna Bridget Department of Clinical Investigation, William Beaumont Army Medical Center, 79920-5001, El Paso, Texas, USA aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/1(1990-02-01), 93-107 0360-3997 14:1<93 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer