caa a22 4500 46576391X CHVBK 20180323111908.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00914027 doi (NATIONALLICENCE)springer-10.1007/BF00914027 Dynamics of avian inflammatory response to cross-linked dextran [Elektronische Daten] Changes in avian blood leukocyte populations [K. Golemboski, S. Bloom, R. Dietert] A model inflammatory compound, cross-linked dextran (Sephadex), which preferentially recruits macrophages, was used to examine shifts in chicken leukocyte distribution and monocyte functional status during the inflammatory response. Following intraperitoneal Sephadex administration, total leukocyte counts were transiently elevated because of a fivefold increase in circulating heterophils (granulocytes). While both heterophils and monocytes may become demarginated, primarily monocytes enter the peritoneal cavity in response to Sephadex. Since leukocyte chemotaxis is an important feature of the inflammatory response, this parameter was analyzed during the recruitment period. The optimum conditions for in vitro chicken mononuclear leukocyte chemotaxis toN-formyl-methionyl-leucyl-phenylalanine (FMLP) were defined and compared with those for the chicken macrophagelike cell line, HD11. Mononuclear leukocytes from birds injected 6 or 12 h previously with Sephadex were significantly decreased in chemotactic ability when compared with controls; at 24 h after injection, the response to FMLP had returned to normal. This system offers an opportunity to examine differential effects of inflammatory stimuli upon leukocyte function and distribution. Plenum Publishing Corporation, 1990 Golemboski K. Institute for Comparative and Environmental Toxicology and Department of Poultry and Avian Sciences, Cornell University, Rice Hall, 14853, Ithaca, New York aut Bloom S. Institute for Comparative and Environmental Toxicology and Department of Poultry and Avian Sciences, Cornell University, Rice Hall, 14853, Ithaca, New York aut Dietert R. Institute for Comparative and Environmental Toxicology and Department of Poultry and Avian Sciences, Cornell University, Rice Hall, 14853, Ithaca, New York aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/1(1990-02-01), 31-40 0360-3997 14:1<31 1990 14 10753 https://doi.org/10.1007/BF00914027 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914027 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Golemboski K. Institute for Comparative and Environmental Toxicology and Department of Poultry and Avian Sciences, Cornell University, Rice Hall, 14853, Ithaca, New York aut NATIONALLICENCE 700 1- Bloom S. Institute for Comparative and Environmental Toxicology and Department of Poultry and Avian Sciences, Cornell University, Rice Hall, 14853, Ithaca, New York aut NATIONALLICENCE 700 1- Dietert R. Institute for Comparative and Environmental Toxicology and Department of Poultry and Avian Sciences, Cornell University, Rice Hall, 14853, Ithaca, New York aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/1(1990-02-01), 31-40 0360-3997 14:1<31 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer