caa a22 4500 465763944 CHVBK 20180323111908.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00914032 doi (NATIONALLICENCE)springer-10.1007/BF00914032 Recombinant human granulocytemacrophage colony-stimulating factor induces granule exocytosis from human polymorphonuclear neutrophils [Elektronische Daten] [Robert Smith, James Justen, Laurel Sam] Exposure of human polymorphonuclear neutrophils (PMNs) to recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) resulted in a time- and concentration-dependent (3-100 units/ml) extracellular release of a specific (vitamin B12-binding protein) but not azurophil granule constituent (myeloperoxidase). Negligible granule exocytosis occurred if PMNs were not preincubated with cytochalasin B prior to contact with GM-CSF. The extent of degranulation elicited with GM-CSF was reduced but not abolished when PMNs were incubated with EGTA in calcium-free medium. GM-CSF did not stimulate a rise in the cytosolic-free calcium concentration ([Ca2+],), and it had no effect on PMN protein kinase C (PKC) activity. Plenum Publishing Corporation, 1990 Smith Robert Department of Hypersensitivity Diseases Research, The Upjohn Company, 49008, Kalamazoo, Michigan aut Justen James Department of Hypersensitivity Diseases Research, The Upjohn Company, 49008, Kalamazoo, Michigan aut Sam Laurel Department of Hypersensitivity Diseases Research, The Upjohn Company, 49008, Kalamazoo, Michigan aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/1(1990-02-01), 83-92 0360-3997 14:1<83 1990 14 10753 https://doi.org/10.1007/BF00914032 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914032 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith Robert Department of Hypersensitivity Diseases Research, The Upjohn Company, 49008, Kalamazoo, Michigan aut NATIONALLICENCE 700 1- Justen James Department of Hypersensitivity Diseases Research, The Upjohn Company, 49008, Kalamazoo, Michigan aut NATIONALLICENCE 700 1- Sam Laurel Department of Hypersensitivity Diseases Research, The Upjohn Company, 49008, Kalamazoo, Michigan aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/1(1990-02-01), 83-92 0360-3997 14:1<83 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer