caa a22 4500 465763987 CHVBK 20180323111908.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00914034 doi (NATIONALLICENCE)springer-10.1007/BF00914034 Chemotactic competence of neutrophils from neonatal calves [Elektronische Daten] Functional comparison with neutrophils from adult cattle [Roland Zwahlen, Daniel Roth] Neonates demonstrate an increased susceptibility to infection. Defects in locomotory functions of newborn neutrophils may play a crucial role in this context. We therefore compared the migratory response of newborn (N-PMN) and adult (APMN) bovine neutrophils in a microwell filter assay. Stimulation with four different endotoxins (E. coli O128 B∶:4 and O55 B∶5;S. abortus equi; S. typhimurium), with zymosan-activated plasma (ZAP) and with C5a induced dose-dependent migration of A-PMNs and N-PMNs. Migration of unstimulated cells and of cells stimulated with diluted ZAP or C5a was higher (P < 0.05) in N-PMNs. Migration of A- and N-PMNs towards C5a was inhibited (P < 0.001) by preincubation with either a steroidal (122μM flumethasone) or nonsteroidal (3.3μM phenylbutazone) antiinflammatory drug. Migratory responses of N-PMNs were inhibited less by SAIDs than were responses of A-PMNs (P < 0.05); indeed dexamethasone slightly enhanced N-PMN responses towards C5a, and 510μM flunixin meglumine enhanced C5a-induced migration in both age groups. Endotoxins fromE. coli O55:B4,S. abortus equi, and S. typhimurium induced a higher rate of migration (P < 0.05) in N-PMNs. In contrast to the above findings, measurement of the maximal distance of migration by the leading-front method did not reveal age-related differences. Migration speed of PMNs was lower after stimulation with C5a than with ZAP, but could be restored partly by adding human vitamin D-binding protein (Gc-globulin). The demonstrated hyperirritability of bovine N-PMNs represents a major functional difference to neonatal neutrophils from other species, including man. It may additionally be related to altered PMN functions and neonatal disease susceptibility. Plenum Publishing Corporation, 1990 Zwahlen Roland Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut Roth Daniel Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/1(1990-02-01), 109-123 0360-3997 14:1<109 1990 14 10753 https://doi.org/10.1007/BF00914034 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914034 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zwahlen Roland Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut NATIONALLICENCE 700 1- Roth Daniel Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/1(1990-02-01), 109-123 0360-3997 14:1<109 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer