caa a22 4500 465764010 CHVBK 20180323111908.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00916372 doi (NATIONALLICENCE)springer-10.1007/BF00916372 Migration of dog polymorphonuclear neutrophilic leukocytes to formylated peptides [Elektronische Daten] [Diana Linnekin, Charles Bowles, Genesio Murano, Thomas MacVittie] Formylated peptides are potent stimulants of polymorphonuclear neutrophilic leukyocyte (PMN) migration from species such as humans and rabbits. Interestingly, PMNs from dogs, cats, pigs and cows have been reported as refractory toN-formyl-1-methionyl-1-leucyl-1-phenylalanine (FMLP) and generally are believed not to express formylpeptide receptors. Formylpeptides are a major component of conditioned media fromE. coli cultures and believed to be a significant element in inflammatory responses elicited byE. coli. Our studies have found thatE. coli filtrate was a potent stimulant of dog PMN migration. Inhibition of migration toE. coli filtrates by the antagonistt-botyloxycarbonyl-1-methionyl-1-leucyl-1-phenylalanine (t-boc-MLP) demonstrated that the migration was mediated through the formylated peptide receptor. Migration in response to peptides with higher affinity for the formylpeptide receptor than FMLP was further evidence for these receptors on the dog PMN. PMNs from dogs migrated in response to FMLP at high concentrations (100μM); however, pretreatment with phorbol myristate acetate resulted in increased migration of dog PMNs in response to concentrations of FMLP as low as 1 pM. These results demonstrate that dog PMNs are responsive to formylpeptides and that these responses can be up-regulated by PMA. Thus PMNs from a species previously thought incapable of responding to formylpeptides can respond to formylpeptide analogs with high affinity for the receptor as well as be primed for enhanced migration to FMLP by PMA. Plenum Publishing Corporation, 1990 Linnekin Diana Department of Physiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland aut Bowles Charles Merrifield Laboratories, Fairfax, Virginia aut Murano Genesio Office of Biologics, Food and Drug Administration, Bethesda, Maryland aut MacVittie Thomas Department of Experimental Hematology, Armed Forces Radiobiology Research Institute, Bethesda, Maryland aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/6(1990-12-01), 691-703 0360-3997 14:6<691 1990 14 10753 https://doi.org/10.1007/BF00916372 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00916372 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Linnekin Diana Department of Physiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland aut NATIONALLICENCE 700 1- Bowles Charles Merrifield Laboratories, Fairfax, Virginia aut NATIONALLICENCE 700 1- Murano Genesio Office of Biologics, Food and Drug Administration, Bethesda, Maryland aut NATIONALLICENCE 700 1- MacVittie Thomas Department of Experimental Hematology, Armed Forces Radiobiology Research Institute, Bethesda, Maryland aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/6(1990-12-01), 691-703 0360-3997 14:6<691 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer