caa a22 4500 465764029 CHVBK 20180323111908.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00916366 doi (NATIONALLICENCE)springer-10.1007/BF00916366 Effect of immunoglobulins on mineral dust-induced production of reactive oxygen metabolites by human macrophages [Elektronische Daten] [Peter Nyberg, Matti Klockars] Mineral dust-induced production of reactive oxygen metabolites (ROMs) by human monocyte-derived macrophages was investigated using lucigenin-dependent chemiluminescence. Chrysotile asbestos alone caused only weak ROM production by macrophages, but the addition of polyclonal immunoglobulin enhanced the reaction strongly. The phenomenon was seen with 1-, 4-, and 7-day-old cell cultures. Polyclonal immunoglobulin also slightly enhanced the ROM responses induced by amosite, crocidolite, and quartz dust. The enhancing effect could be achieved with several monoclonal immunoglobulins (isolated from the sera of myeloma patients), but IgA and IgG had the strongest effects. We suggest that immunoglobulins may interact with mineral dusts in a "nonimmunological,” antigen-independent way and that the so-formed dust-immunoglobulin complexes may amplify the production of ROMs by inflammatory cells. This may explain a number of in vivo phenomena in which immune responses (for instance hypergammaglobulinemia and the presence of autoantibodies) have been shown to relate to the progression of mineral dust-induced pulmonary disease. Plenum Publishing Corporation, 1990 Nyberg Peter Institute of Occupational Health, Topeliuksenkatu 41 a A, SF-00250, Helsinki, Finland aut Klockars Matti Institute of Occupational Health, Topeliuksenkatu 41 a A, SF-00250, Helsinki, Finland aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/6(1990-12-01), 621-629 0360-3997 14:6<621 1990 14 10753 https://doi.org/10.1007/BF00916366 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00916366 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nyberg Peter Institute of Occupational Health, Topeliuksenkatu 41 a A, SF-00250, Helsinki, Finland aut NATIONALLICENCE 700 1- Klockars Matti Institute of Occupational Health, Topeliuksenkatu 41 a A, SF-00250, Helsinki, Finland aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/6(1990-12-01), 621-629 0360-3997 14:6<621 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer