caa a22 4500 465764061 CHVBK 20180323111908.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00916373 doi (NATIONALLICENCE)springer-10.1007/BF00916373 Effect of phagocytosis of erythrocytes and erythrocyte ghosts on macrophage phagocytic function and hydrogen peroxide production [Elektronische Daten] [Laura Commins, Daniel Loegering, Paul Gudewicz] Our previous studies have shown that an in vivo phagocytic challenge with IgG-coated erythrocytes can depress Kupffer cell complement and Fc receptor function, as well as decrease the survival rate following endotoxemia and bacteremia. In an effort to better understand the mechanism underlying these in vivo findings, the present study evaluated the in vitro effects of a phagocytic challenge with either IgG-coated erythrocytes (EIgG) or erythrocyte ghosts (GIgG) on macrophage phagocytic and respiratory burst activity. Elicited rat peritoneal macrophage (PM) monolayers were challenged with varying doses of EIgG, then the noninternalized EIgG were lysed hypotonically and the monolayers incubated for an additional hour prior to determining phagocytic function and PMA-stimulated hydrogen peroxide production. Challenge of PM with 1×106 EIgG per well had no effect, but challenge with 1×107 or 1×108 EIgG per well caused a dose-dependent depression of phagocytic function or hydrogen peroxide production. GIgG were formed by hypotonically lysing EIgG bound to PM at 4°C. The bound GIgG were phagocytized during a subsequent incubation at 37°C. Challenge with GIgG depressed phagocytic function only with the highest challenge dose tested (1×108 per well) and did not depress hydrogen peroxide production. The observation that prior phagocytic challenge with EIgG depressed macrophage function to a greater extent than challenge with GIgG supports our previous in vivo observations. Furthermore, these studies suggest that the internalization of erythrocyte contents, and not phagocytosis per se, plays an important role in determining macrophage host defense function. Plenum Publishing Corporation, 1990 Commins Laura Department of Physiology and Cell Biology, Albany Medical College, 47 New Scotland Avenue, 12208, Albany, New York aut Loegering Daniel Department of Physiology and Cell Biology, Albany Medical College, 47 New Scotland Avenue, 12208, Albany, New York aut Gudewicz Paul Department of Physiology and Cell Biology, Albany Medical College, 47 New Scotland Avenue, 12208, Albany, New York aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/6(1990-12-01), 705-716 0360-3997 14:6<705 1990 14 10753 https://doi.org/10.1007/BF00916373 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00916373 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Commins Laura Department of Physiology and Cell Biology, Albany Medical College, 47 New Scotland Avenue, 12208, Albany, New York aut NATIONALLICENCE 700 1- Loegering Daniel Department of Physiology and Cell Biology, Albany Medical College, 47 New Scotland Avenue, 12208, Albany, New York aut NATIONALLICENCE 700 1- Gudewicz Paul Department of Physiology and Cell Biology, Albany Medical College, 47 New Scotland Avenue, 12208, Albany, New York aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/6(1990-12-01), 705-716 0360-3997 14:6<705 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer