caa a22 4500 465764193 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00915813 doi (NATIONALLICENCE)springer-10.1007/BF00915813 Effect of sodium azide on hydrogen peroxide production by zymosan-activated human neutrophils [Elektronische Daten] [A. Nahum, M. Hegarty, H. Chen, W. Chamberlin, J. Sznajder] Stimulated neutrophils (PMNs) produce large quantities of superoxide anion, which is the precursor for hydrogen peroxide (H2O2). We developed a new fluorimetric assay to measure the H2O2 released by zymosan A-activated PMNs utilizing the oxidation ofp-hydroxyphenylacetic acid by H2O2 to its fluorescent dimer in the presence of horseradish peroxidase. Zymosan-activated PMNs isolated from nine healthy volunteers and 20 patients with acute hypoxemic respiratory failure (AHRF) released after 90 min 2.3±0.3 and 2.4±1.3 nmol H2O2/106 PMNs, respectively. Inhibition of the heme enzymes by 1.0 mM sodium azide (NaN3) increased the H2O2 production to 21.6±4.4 nmol H2O2/106 PMNs in the control group (P<0.001), and to 22.5±14.7 nmol H2O2/106 PMNs in patients with AHRF (P<0.001). Incubation temperature, room temperature or 37‡C, did not change the total amount of H2O2 produced after 90 min by zymosan-activated PMN. Addition of NaN3 improved both the sensitivity and reproducibility of the measurement of H2O2 and allowed detection of H2O2 released by PMNs with coefficients of variation of less than 5% at PMN concentrations as low as 0.1×106 cells/ml. The amount of H2O2 released by activated PMNs did not distinguish healthy controls from patients with AHRF. Plenum Publishing Corporation, 1990 Nahum A. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut Hegarty M. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut Chen H. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut Chamberlin W. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut Sznajder J. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/3(1990-06-01), 285-296 0360-3997 14:3<285 1990 14 10753 https://doi.org/10.1007/BF00915813 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00915813 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nahum A. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut NATIONALLICENCE 700 1- Hegarty M. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut NATIONALLICENCE 700 1- Chen H. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut NATIONALLICENCE 700 1- Chamberlin W. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut NATIONALLICENCE 700 1- Sznajder J. Section of Pulmonary and Critical Care Medicine Michael Reese Hospital and Medical Center, USA aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/3(1990-06-01), 285-296 0360-3997 14:3<285 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer