caa a22 4500 465764215 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00914092 doi (NATIONALLICENCE)springer-10.1007/BF00914092 Reactivity of active center analogs of Cu2Zn2 superoxide dismutase on activated polymorphonuclear leukocytes [Elektronische Daten] [Ralf Miesel, Hans-Jürgen Hartmann, Yuejin Li, Ulrich Weser] In unseparated human blood the Cu2Zn2 superoxide dismutase mimetic reactivity of several differently coordinated low Mr copper chelates on TPA-activated polymorphonuclear leukocytes was evaluated and compared to their apo-chelates, CuSO4, and the native enzyme. Similar to intact superoxide dismutase, 350-400 nM Cu flexibly complexed in a di-Schiff base mode in CuPu(Py)2 and CuPu(Im)2, respectively, was sufficient to inhibit the oxidative burst-dependent superoxide production of human blood phagocytes by 50%. Acetate-or biuret-type copper chelates behaved like CuSO2. The catalytic superoxide dismuting reactivity of the di-Schiff base active center analogs of SOD was confirmed using isolated porcine PMNs. Even in the presence of 600μM albumin as a model for competitive copper chelation in biological fluids CuPu(Py)2 and CuPu(Im)2 remained active. The stability during the Cu(I)/Cu(II) redox cycling was demonstrated in the presence of activated PMNs and albumin, taking advantage of the electron paramagnetic properties of CuPu(Py)2 and CuPu(Im)2. Plenum Publishing Corporation, 1990 Miesel Ralf Anorganische Biochemie Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 7400, Tübingen, F.R.G. aut Hartmann Hans-Jürgen Anorganische Biochemie Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 7400, Tübingen, F.R.G. aut Li Yuejin Anorganische Biochemie Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 7400, Tübingen, F.R.G. aut Weser Ulrich Anorganische Biochemie Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 7400, Tübingen, F.R.G. aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 409-419 0360-3997 14:4<409 1990 14 10753 https://doi.org/10.1007/BF00914092 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914092 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miesel Ralf Anorganische Biochemie Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 7400, Tübingen, F.R.G aut NATIONALLICENCE 700 1- Hartmann Hans-Jürgen Anorganische Biochemie Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 7400, Tübingen, F.R.G aut NATIONALLICENCE 700 1- Li Yuejin Anorganische Biochemie Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 7400, Tübingen, F.R.G aut NATIONALLICENCE 700 1- Weser Ulrich Anorganische Biochemie Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler Strasse 4, 7400, Tübingen, F.R.G aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 409-419 0360-3997 14:4<409 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer