caa a22 4500 465764223 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00914094 doi (NATIONALLICENCE)springer-10.1007/BF00914094 An enzyme-assessed microplate-assay for neutrophil adherence [Elektronische Daten] I. IgA-Induced adherence of human PMNs [W. Bullock, M. Rogers, W. Gabler, H. Creamer] The binding of PMNs to extracellular matrix and cells is crucial to PMN host defense. Adherence mechanisms and the many families of molecules involved are major areas of study. We present here details of an enzyme-assessed microtiter plate assay for neutrophil adherence. This assay uses low numbers of cells (50,000/well) and permits analysis of several hundred wells in a short period of time, by using an ELISA reader. With this assay we observed 5-to 10-fold increases in the number of adherent human PMNs in response to nanogram amounts of LPS or as little as 5.0μg/ml of aggregated IgA. Although fluoride blocked the LPS-induced adherence response, IgA-induced cell binding was largely unaffected. Plenum Publishing Corporation, 1990 Bullock W. Department of Oral Microbiology/Immunology, Oregon Health Sciences University, 611 SW Campus Drive, 97201, Portland, Oregon aut Rogers M. Department of Oral Microbiology/Immunology, Oregon Health Sciences University, 611 SW Campus Drive, 97201, Portland, Oregon aut Gabler W. Department of Biochemistry School of Dentistry, Oregon Health Sciences University, 611 SW Campus Drive, 97201, Portland, Oregon aut Creamer H. Department of Oral Microbiology/Immunology, Oregon Health Sciences University, 611 SW Campus Drive, 97201, Portland, Oregon aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 427-445 0360-3997 14:4<427 1990 14 10753 https://doi.org/10.1007/BF00914094 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914094 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bullock W. Department of Oral Microbiology/Immunology, Oregon Health Sciences University, 611 SW Campus Drive, 97201, Portland, Oregon aut NATIONALLICENCE 700 1- Rogers M. Department of Oral Microbiology/Immunology, Oregon Health Sciences University, 611 SW Campus Drive, 97201, Portland, Oregon aut NATIONALLICENCE 700 1- Gabler W. Department of Biochemistry School of Dentistry, Oregon Health Sciences University, 611 SW Campus Drive, 97201, Portland, Oregon aut NATIONALLICENCE 700 1- Creamer H. Department of Oral Microbiology/Immunology, Oregon Health Sciences University, 611 SW Campus Drive, 97201, Portland, Oregon aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 427-445 0360-3997 14:4<427 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer