caa a22 4500 46576424X CHVBK 20180323111909.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00914089 doi (NATIONALLICENCE)springer-10.1007/BF00914089 In vitro aggregation of bovine neonatal neutrophils [Elektronische Daten] A comparative study with adult cattle [Roland Zwahlen, Daniel Roth, Marianne Wyder-Walther] Deficient in vitro functions of neonatal neutrophils have been reported in various species. They may be functionally related to the well-known susceptibility of newborn individuals to microbial infections. To evaluate an early step in the sequence of neutrophil activation, neutrophils from adult cows (A-PMN) and newborn calves (N-PMN) were stimulated with zymosan-activated plasma (ZAP) or with the lipid mediator platelet-activating factor (PAF): Aggregation was recorded kinetically in a standard aggregometer and measured quantitatively as the area under the aggregation curve (AUAC). The mean ±SEM of the AUAC of the first 2.5 min of the reaction induced with ZAP was similar in N-PMN and A-PMN. However, N-PMN deaggregated only partially, whereas A-PMN deaggregated almost completely (P<0.05). This may indicate a mechanism of microvascular sequestration in vivo with the potential to inhibit chemotaxis. PAF (10−5−10−10 M) aggregated N- and A-PMNs similarly and dose-dependently with a maximal reaction at 10−6 M. Inhibition of aggregation induced by 10−6 M PAF was evaluated by preincubation with four antiinflammatory drugs: dexamethasone (Dex: 5.1, 51.0, 510.0μM), flumethasone (Flu: 12.2 and 122.0μM), phenylbutazone (PB: 0.33 and 3.3 mM), and flunixin meglumine (Flxin: 51 and 510μM). Dex and Flu each inhibited (P< 0.05) PAF-induced N-PMN aggregation at the highest dose, and A-PMN aggregation at the two higher doses. PB and Flxin each inhibited aggregation of N- and APMNs at all doses used. We compared the inhibition rate in both age groups and could demonstrate that Dex, Flu, and Flxin each at the highest dose, and PB at all doses used, inhibited PAF-induced aggregation less (P< 0.05) in N-PMNs than in A-PMNs. These functional differences indicate hyperirritability of N-PMNs, and they need further elucidation to help understand mechanisms of increased neonatal susceptibility. Plenum Publishing Corporation, 1990 Zwahlen Roland Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut Roth Daniel Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut Wyder-Walther Marianne Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 375-387 0360-3997 14:4<375 1990 14 10753 https://doi.org/10.1007/BF00914089 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914089 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zwahlen Roland Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut NATIONALLICENCE 700 1- Roth Daniel Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut NATIONALLICENCE 700 1- Wyder-Walther Marianne Institute of Veterinary Pathology, University of Bern, P.O. Box 2735, CH-3001, Bern, Switzerland aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 375-387 0360-3997 14:4<375 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer