caa a22 4500 465764266 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00914090 doi (NATIONALLICENCE)springer-10.1007/BF00914090 Characterization of extracellular phospholipase A2 (PLA2) activity in fluid and peritoneal cells from casein-treated rats [Elektronische Daten] [Theresa Stevens, Michael McGowan, John Giannaras, Janet Kerr] Extracellular phospholipase A2 activity (PLA2) found in the fluid and cells of the peritoneal cavity of rats injected with casein is described. PLA2 activities from both the fluid and cells require Ca2+ and have pH optima of 7. Acid-extraction increased PLA2 activity in the polymorphonuclear leukocyte (PMN) homogenates 20-fold but not the PLA2 activity in the extracellular fluid. Acid extraction also increased the sensitivity of the PLA2 activities to standard inhibitors. Since the PLA2 activities described in this model have characteristics similar to other inflammatory PLA2s, including human synovial fluid PLA2, casein stimulation should prove useful for testing potential inhibitors. Plenum Publishing Corporation, 1990 Stevens Theresa Medical Products Department, E. I. du Pont de Nemours & Co., Inc. Experimental Station, E400/4223, P. O. Box 80400, 19880-0400, Wilmington, Delaware aut McGowan Michael Medical Products Department, E. I. du Pont de Nemours & Co., Inc. Experimental Station, E400/4223, P. O. Box 80400, 19880-0400, Wilmington, Delaware aut Giannaras John Medical Products Department, E. I. du Pont de Nemours & Co., Inc. Experimental Station, E400/4223, P. O. Box 80400, 19880-0400, Wilmington, Delaware aut Kerr Janet Medical Products Department, E. I. du Pont de Nemours & Co., Inc. Experimental Station, E400/4223, P. O. Box 80400, 19880-0400, Wilmington, Delaware aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 389-399 0360-3997 14:4<389 1990 14 10753 https://doi.org/10.1007/BF00914090 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914090 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stevens Theresa Medical Products Department, E. I. du Pont de Nemours & Co., Inc. Experimental Station, E400/4223, P. O. Box 80400, 19880-0400, Wilmington, Delaware aut NATIONALLICENCE 700 1- McGowan Michael Medical Products Department, E. I. du Pont de Nemours & Co., Inc. Experimental Station, E400/4223, P. O. Box 80400, 19880-0400, Wilmington, Delaware aut NATIONALLICENCE 700 1- Giannaras John Medical Products Department, E. I. du Pont de Nemours & Co., Inc. Experimental Station, E400/4223, P. O. Box 80400, 19880-0400, Wilmington, Delaware aut NATIONALLICENCE 700 1- Kerr Janet Medical Products Department, E. I. du Pont de Nemours & Co., Inc. Experimental Station, E400/4223, P. O. Box 80400, 19880-0400, Wilmington, Delaware aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 389-399 0360-3997 14:4<389 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer