caa a22 4500 465764274 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00914096 doi (NATIONALLICENCE)springer-10.1007/BF00914096 Myeloperoxidase in human lung lavage [Elektronische Daten] II. Internalization of myeloperoxidase by alveolar macrophages [Birgitta Schmekel, Yngve Hörnblad, Margareta Linden, Christer Sundström, Per Venge] Bronchial wash and bronchoalveolar lavage were performed in 12 healthy subjects (five smokers), in order to elucidate whether or not material of neutrophil origin may be phagocytized by lung macrophages in vivo. Cells from different levels in the bronchial tree were obtained by sequential injection and subsequent aspiration of either four 50-ml or five 10-ml aliquots. Each aliquot was used for the determination of total and differential cell counts. The proportion of myeloperoxidase-positive alveolar macrophages was determined by specific immune histochemical staining. The percentage of myeloperoxidase-positive macrophages was highest (median 94.8%, range 37-98.5%) in the 10-ml aliquots and lowest in the last three 50-ml aliquots (median values 1-2.5%) (P < 0.001). A significant correlation was obtained between the fraction of myeloperoxidase-positive macrophages and the percentage count of bronchoalveolar lavage neutrophils (r=0.466,P < 0.05). Furthermore, the cellular myeloperoxidase showed a significant inverse correlation (r=−0.46,P < 0.05) to the viability of the bronchoalveolar lavage cells. Our findings are compatible with previous demonstrations in animals of neutrophil phagocytosis by lung macrophages and show that this phenomenon in particular occurs in the more proximal airways. The internalization of neutrophils or neutrophil components by airway macrophages may be an important scavenger mechanism for protection of the lung from the deleterious effects of activated neutrophils. Plenum Publishing Corporation, 1990 Schmekel Birgitta Department of Explorative Clinical Research, AB Draco, Lund, Sweden aut Hörnblad Yngve Department of Lung Medicine, University Hospital, Lund, Sweden aut Linden Margareta Department of Explorative Clinical Research, AB Draco, Lund, Sweden aut Sundström Christer Department of Pathology, University Hospital, Uppsala, Sweden aut Venge Per Department of Clinical Chemistry, University Hospital, Uppsala, Sweden aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 455-461 0360-3997 14:4<455 1990 14 10753 https://doi.org/10.1007/BF00914096 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914096 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmekel Birgitta Department of Explorative Clinical Research, AB Draco, Lund, Sweden aut NATIONALLICENCE 700 1- Hörnblad Yngve Department of Lung Medicine, University Hospital, Lund, Sweden aut NATIONALLICENCE 700 1- Linden Margareta Department of Explorative Clinical Research, AB Draco, Lund, Sweden aut NATIONALLICENCE 700 1- Sundström Christer Department of Pathology, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 700 1- Venge Per Department of Clinical Chemistry, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 455-461 0360-3997 14:4<455 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer