caa a22 4500 465764290 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00914095 doi (NATIONALLICENCE)springer-10.1007/BF00914095 Myeloperoxidase in human lung lavage [Elektronische Daten] I. A marker of local neutrophil activity [Birgitta Schmekel, S. Karlsson, M. Linden, C. Sundström, H. Tegner, P. Venge] The origin of myeloperoxidase (MPO) in bronchoalveolar lavage (BAL) was investigated in the first part of the study. Radioimmunoassay of the cellular and supernatant MPO content as well as the peroxidase-antiperoxidase (PAP) technique were employed to determine the cellular source of MPO. The concentrations of MPO were measured in serum and BAL in the second part of the study. The aim was to determine whether the capillary bed was also a source of MPO. Neutrophil numbers in BALs obtained from 20 healthy subjects correlated significantly to the concentrations of MPO in cell-free BAL supernatans (r=0.643,P≤0.01). The cellular content of MPO in mixed BAL cells was significantly correlated to the number of neutrophils in the mixture (r=0.536,P<0.05), but not to the number of any other cells. Moreover, the PAP-technique identified MPO in lung tissue neutrophils in resection specimens obtained from three patients undergoing surgery. This technique also revealed strong MPO activity in all BAL neutrophils and a weak activity in merely 4% of the alveolar macrophages in cytospin preparations obtained from seven BALs. High BAL/serum ratio of MPO concentrations suggests that MPO is of local origin, rather than passively diffused from the circulating pool. We therefore conclude that strong evidence suggests that MPO in BAL originates from lung neutrophils and that BAL MPO content may be used to estimate the neutrophil presence or activation in epithelium lining fluid. Plenum Publishing Corporation, 1990 Schmekel Birgitta Department of Explorative Clinical Research, AB Draco, Lund, Sweden aut Karlsson S. Department of Lung Medicine, University Hospital, Lund, Sweden aut Linden M. Department of Explorative Clinical Research, AB Draco, Lund, Sweden aut Sundström C. Department of Pathology, University Hospital, Uppsala, Sweden aut Tegner H. Department of Otorhinolaryngology, University Hospital, Malmö, Sweden aut Venge P. Department of Clinical Chemistry, University Hospital, Uppsala, Sweden aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 447-454 0360-3997 14:4<447 1990 14 10753 https://doi.org/10.1007/BF00914095 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914095 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmekel Birgitta Department of Explorative Clinical Research, AB Draco, Lund, Sweden aut NATIONALLICENCE 700 1- Karlsson S. Department of Lung Medicine, University Hospital, Lund, Sweden aut NATIONALLICENCE 700 1- Linden M. Department of Explorative Clinical Research, AB Draco, Lund, Sweden aut NATIONALLICENCE 700 1- Sundström C. Department of Pathology, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 700 1- Tegner H. Department of Otorhinolaryngology, University Hospital, Malmö, Sweden aut NATIONALLICENCE 700 1- Venge P. Department of Clinical Chemistry, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 447-454 0360-3997 14:4<447 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer