caa a22 4500 465764304 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00914093 doi (NATIONALLICENCE)springer-10.1007/BF00914093 Identification of β 2-adrenoceptors on guinea pig alveolar macrophages using (−)-3-[125I]iodocyanopindolol [Elektronische Daten] [R. Leurs, F. Beusenberg, A. Bast, J. van Amsterdam, H. Timmerman] The β-adrenoceptor antagonist (−)-3-[125I]iodocyanopindolol ([125I]ICYP) binds with high affinity and in a saturable way to membranes of guinea pig alveolar macrophages. The equilibrium dissociation constant for [125I]ICYP is 24.3 ± 1.2 pM, and the number of binding sites is 166.3 ± 13.7 fmol/mg protein (N=4, ±SEM). Displacement studies with selective antagonists showed that [125I]ICYP labels β2-adrenoceptors on guinea pig alveolar macrophages. Plenum Publishing Corporation, 1990 Leurs R. Department of Pharmacochemistry Faculty of Chemistry, Vrije Universiteit, de Boelelaan 1083, 1081, HV Amsterdam, The Netherlands aut Beusenberg F. Department of Pharmacology Faculty of Medicine, Erasmus University Rotterdam, PO Box 1738, 3000, DR Rotterdam, The Netherlands aut Bast A. Department of Pharmacochemistry Faculty of Chemistry, Vrije Universiteit, de Boelelaan 1083, 1081, HV Amsterdam, The Netherlands aut van Amsterdam J. Department of Pharmacology Faculty of Medicine, Erasmus University Rotterdam, PO Box 1738, 3000, DR Rotterdam, The Netherlands aut Timmerman H. Department of Pharmacochemistry Faculty of Chemistry, Vrije Universiteit, de Boelelaan 1083, 1081, HV Amsterdam, The Netherlands aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 421-426 0360-3997 14:4<421 1990 14 10753 https://doi.org/10.1007/BF00914093 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914093 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leurs R. Department of Pharmacochemistry Faculty of Chemistry, Vrije Universiteit, de Boelelaan 1083, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Beusenberg F. Department of Pharmacology Faculty of Medicine, Erasmus University Rotterdam, PO Box 1738, 3000, DR Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Bast A. Department of Pharmacochemistry Faculty of Chemistry, Vrije Universiteit, de Boelelaan 1083, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- van Amsterdam J. Department of Pharmacology Faculty of Medicine, Erasmus University Rotterdam, PO Box 1738, 3000, DR Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Timmerman H. Department of Pharmacochemistry Faculty of Chemistry, Vrije Universiteit, de Boelelaan 1083, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/4(1990-08-01), 421-426 0360-3997 14:4<421 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer