caa a22 4500 465764320 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00914270 doi (NATIONALLICENCE)springer-10.1007/BF00914270 Transforming growth factor Β1 influences glycosylation of α1-protease inhibitor in human hepatoma cell lines [Elektronische Daten] [Andrzej Mackiewicz, Irving Kushner] We have previously shown that changes in acute-phase protein glycosylation result from alterations occurring within hepatocytes as a result of regulation by cytokines, that the glycosylation patterns of proteins secreted by Hep 3B and Hep G2 cells respond differently to the crude mixtures of cytokines found in conditioned medium from LPS-stimulated monocytes, and that interleukin-6 (IL-6) causes increased concanavalin A (Con A) binding ofαl protease inhibitor in Hep 3B cells and decreased Con A binding of this protein in Hep G2 cells. In the present study we found that transforming growth factorΒl (TGF-Β), like IL-6, led to secretion of forms ofαl-protease inhibitor with increased Con A binding in Hep 3B cells, and that IL-6 and TGF-Β in combination were additive. In contrast, in Hep G2 cells, TGF-Β had an effect opposite to that produced by IL-6, leading to secretion of forms ofαl-protease inhibitor with increased Con A binding. When employed in combination with IL-6, TGF-Β abolished the effect of that cytokine. These studies indicate that TGF-Β influences glycosylation of al-protease inhibitor in two human hepatoma cell lines in a manner that can be differentiated from that of IL-6. The identification of TGF-Β as a second defined cytokine capable of influencing glycoprotein glycosylation and the demonstration that the effect of one cytokine can be modulated by another cytokine support the view that changes in glycosylation of plasma proteins are mediated by combinations of cytokines. Plenum Publishing Corporation, 1990 Mackiewicz Andrzej Laboratory of Immunochemistry Department of Immunology and Rheumatology, Academy of Medicine, 61626, Poznan, Poland aut Kushner Irving Department of Medicine Case Western Reserve, University at MetroHealth Medical Center, 44109, Cleveland, Ohio aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 485-497 0360-3997 14:5<485 1990 14 10753 https://doi.org/10.1007/BF00914270 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914270 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mackiewicz Andrzej Laboratory of Immunochemistry Department of Immunology and Rheumatology, Academy of Medicine, 61626, Poznan, Poland aut NATIONALLICENCE 700 1- Kushner Irving Department of Medicine Case Western Reserve, University at MetroHealth Medical Center, 44109, Cleveland, Ohio aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 485-497 0360-3997 14:5<485 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer