caa a22 4500 465764347 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00914271 doi (NATIONALLICENCE)springer-10.1007/BF00914271 A monoclonal antibody-defined adhesion-related antigen on bovine neutrophils is required for neutrophil aggregation [Elektronische Daten] [Philip Bochsler, Monique Doré, Nancy Neilsen, David Slauson] Surface adhesion molecules present on human leukocytes are known to regulate certain adhesion-related events, such as adhesion to endothelium, extravasation, and aggregation. We have used a mouse anti-human monoclonal antibody designated 60.3 (MAb 60.3) and indirect immunofluorescence technique to identify an antigen on bovine neutrophils (PMNs). MAb 60.3 bound to resting and stimulated bovine PMN in a surface-oriented pattern. Immunofluorescence flow cytometric analysis indicated that wanning the PMNs from 4‡C to 37‡C slightly increased (13.9%) expression of the antigen recognized by MAb 60.3. Zymosan-activated serum (ZAS, 10%) increased antigen expression by 12.4% over those PMNs in buffer alone, and phorbol 12-myristate 13-acetate (PMA; 100 ng/ml) by 65.6%. Bacterial lipopolysaccharide (LPS; 1Μg/ml) fromE. coli 0111:B4 did not enhance antigen expression. The functional nature of this antigen was demonstrated by use of MAb 60.3 and PMN aggregation. Preincubation of bovine PMN with MAb 60.3 for 10 min resulted in nearly complete inhibition of PMN-PMN aggregation upon subsequent stimulation with PMA (100 ng/ml); preincubation with a control antibody did not inhibit aggregation. These results indicate that bovine PMNs possess surface molecule(s) that may function in adhesion-related events, and surface expression may be enhanced by PMN stimulation. Plenum Publishing Corporation, 1990 Bochsler Philip Inflammation Research Laboratory Department of Pathology College of Veterinary Medicine, Cornell University, Ithaca, New York aut Doré Monique Inflammation Research Laboratory Department of Pathology College of Veterinary Medicine, Cornell University, Ithaca, New York aut Neilsen Nancy Inflammation Research Laboratory Department of Pathology College of Veterinary Medicine, Cornell University, Ithaca, New York aut Slauson David Inflammation Research Laboratory Department of Pathology College of Veterinary Medicine, Cornell University, Ithaca, New York aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 499-508 0360-3997 14:5<499 1990 14 10753 https://doi.org/10.1007/BF00914271 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914271 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bochsler Philip Inflammation Research Laboratory Department of Pathology College of Veterinary Medicine, Cornell University, Ithaca, New York aut NATIONALLICENCE 700 1- Doré Monique Inflammation Research Laboratory Department of Pathology College of Veterinary Medicine, Cornell University, Ithaca, New York aut NATIONALLICENCE 700 1- Neilsen Nancy Inflammation Research Laboratory Department of Pathology College of Veterinary Medicine, Cornell University, Ithaca, New York aut NATIONALLICENCE 700 1- Slauson David Inflammation Research Laboratory Department of Pathology College of Veterinary Medicine, Cornell University, Ithaca, New York aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 499-508 0360-3997 14:5<499 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer