caa a22 4500 465764371 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00914279 doi (NATIONALLICENCE)springer-10.1007/BF00914279 Bacterial lipopolysaccharide enhances polymorphonuclear leukocyte function independent of changes in intracellular calcium [Elektronische Daten] [Jon Klein, Vaughn Payne, Theresa Schepers, Kenneth McLeish] Bacterial lipopolysaccharide (LPS) enhanced expression of C3bi receptors (CR3), phagocytosis of opsonized bacteria, and subsequent hydrogen peroxide (H2O2) production by human polymorphonuclear leukocytes (PMNs). The role of changes in intracellular calcium concentration ([Ca2+]i) in LPS-induced priming was examined by determining the effect of modulators of intracellular calcium on enhanced PMN function, determining the ability of calcium ionophores to reproduce the effects of LPS, and measuring PMN [Ca2+]i following addition of LPS. Inhibition of intracellular calcium-dependent processes with TMB-8 or quin-2 blocked all three measures of LPS-induced priming. LPS did not stimulate an increase in [Ca2+]i, and calcium ionophores failed to reproduce the effect of LPS. Maintenance of [Ca2+ i is necessary for LPS priming, but an increase in [Ca2+]i is not a component of the signal transduction pathway leading to PMN priming by LPS. Plenum Publishing Corporation, 1990 Klein Jon Department of Medicine, University of Louisville School of Medicine, 40292, Louisville, Kentucky aut Payne Vaughn Department of Medicine, University of Louisville School of Medicine, 40292, Louisville, Kentucky aut Schepers Theresa Department of Biochemistry, University of Louisville School of Medicine, 40292, Louisville, Kentucky aut McLeish Kenneth Department of Medicine, University of Louisville School of Medicine, 40292, Louisville, Kentucky aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 599-611 0360-3997 14:5<599 1990 14 10753 https://doi.org/10.1007/BF00914279 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914279 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Klein Jon Department of Medicine, University of Louisville School of Medicine, 40292, Louisville, Kentucky aut NATIONALLICENCE 700 1- Payne Vaughn Department of Medicine, University of Louisville School of Medicine, 40292, Louisville, Kentucky aut NATIONALLICENCE 700 1- Schepers Theresa Department of Biochemistry, University of Louisville School of Medicine, 40292, Louisville, Kentucky aut NATIONALLICENCE 700 1- McLeish Kenneth Department of Medicine, University of Louisville School of Medicine, 40292, Louisville, Kentucky aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 599-611 0360-3997 14:5<599 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer