caa a22 4500 465764398 CHVBK 20180323111909.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00914274 doi (NATIONALLICENCE)springer-10.1007/BF00914274 Effects of neutrophil-derived oxidants on intestinal permeability, electrolyte transport, and epithelial cell viability [Elektronische Daten] [Matthew Grisham, Timothy Gaginella, Christoph von Ritter, Hiroshi Tamai, Robert Be, D. Granger] There are several pathophysiologic conditions in which intestinal inflammation is associated with enhanced mucosal permeability, fluid loss, and epithelial cell injury. The objective of this study was to determine the effects of polymorphonuclear leukocyte (PMN) -derived oxidants on ileal mucosal permeability in vivo as well as electrolyte transport and epithelial cell viability in vitro. Using blood-tolumen clearance of [51Cr]EDTA as a measure of mucosal permeability, we found that luminal perfusion with hydrogen peroxide (H2O2), hypochlorous acid (HOCl), or monochloramine (NH2Cl) produced a dose-dependent increase in mucosal permeability. Perfusion with 0.1 mM, 0.5 mM, and 1.0 mM oxidant produced a 2±1, 5±2, and 11±5-fold increase in mucosal permeability for H2O2, a 2±1, 8±3, and 36±12-fold increase for HOC1, and a 3±1, 11±2, and 30±7-fold increase for NH2Cl. Taurine monochloramine (TauNHCl) was ineffective in enhancing the blood-to-lumen clearance of [51Cr]EDTA. Furthermore, 0.01 mM and 0.1 mM NH2Cl and H2O2 produced significant increases in short-circuit current across rat ileum in vitro, whereas HOC1 and TauNHCl were without effect. Tissue resistance and potential difference were not altered, suggesting that NH2Cl, HOCl, and H2O2 were not cytotoxic under these conditions. Cultured intestinal epithelial cells exposed to NH2Cl and HOCl were injured in a dose-dependent manner in vitro, whereas H2O2 and TauNHCl were nontoxic. Taken together, our data suggest that PMN-derived oxidants may mediate the enhanced mucosal permeability, electrolyte transport, and epithelial cell injury associated with acute inflammation of the bowel. Plenum Publishing Corporation, 1990 Grisham Matthew Department of Physiology and Biophysics, LSU Medical Center, 71130, Shreveport, Louisiana aut Gaginella Timothy Division of Gastrointestinal Biology Searle Research and Development, G. D. Searle & Company, 60077, Skokie, Illinois aut von Ritter Christoph Department of Physiology and Biophysics, LSU Medical Center, 71130, Shreveport, Louisiana aut Tamai Hiroshi Division of Gastrointestinal Biology Searle Research and Development, G. D. Searle & Company, 60077, Skokie, Illinois aut Be Robert Department of Physiology and Biophysics, LSU Medical Center, 71130, Shreveport, Louisiana aut Granger D. Department of Physiology and Biophysics, LSU Medical Center, 71130, Shreveport, Louisiana aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 531-542 0360-3997 14:5<531 1990 14 10753 https://doi.org/10.1007/BF00914274 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914274 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grisham Matthew Department of Physiology and Biophysics, LSU Medical Center, 71130, Shreveport, Louisiana aut NATIONALLICENCE 700 1- Gaginella Timothy Division of Gastrointestinal Biology Searle Research and Development, G. D. Searle & Company, 60077, Skokie, Illinois aut NATIONALLICENCE 700 1- von Ritter Christoph Department of Physiology and Biophysics, LSU Medical Center, 71130, Shreveport, Louisiana aut NATIONALLICENCE 700 1- Tamai Hiroshi Division of Gastrointestinal Biology Searle Research and Development, G. D. Searle & Company, 60077, Skokie, Illinois aut NATIONALLICENCE 700 1- Be Robert Department of Physiology and Biophysics, LSU Medical Center, 71130, Shreveport, Louisiana aut NATIONALLICENCE 700 1- Granger D. Department of Physiology and Biophysics, LSU Medical Center, 71130, Shreveport, Louisiana aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 531-542 0360-3997 14:5<531 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer