caa a22 4500 46576441X CHVBK 20180323111909.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00914273 doi (NATIONALLICENCE)springer-10.1007/BF00914273 Priming effect of fibronectin on respiratory burst of human neutrophils induced by formyl peptides and platelet-activating factor [Elektronische Daten] [Lena Stanislawski, Trung Huu, Axel Perianin] Fibronectin (FN), a glycoprotein present in the plasma and the extracellular matrix, has been shown to enhance adherence-related functions of polymorphonuclear leukocytes (PMNs). In this study we investigated the effects of FN on the activation of human PMNs in suspension by soluble stimuli, as determined by the generation of Superoxide radicals (respiratory burst). FN (up to 100Μg/ml) did not directly stimulate the PMN respiratory burst assessed using a sensitive assay, luminol-dependent chemiluminescence (CL). Low FN concentrations (Up to 25Μgl ml) caused a dose-dependent enhancement of the CL induced by two chemoattractants,N-formyl-methionyl-leucyl-phenylalanine (FMLP) and platelet-activating factor (Paf), and also by phorbol myristate acetate (PMA), a known protein kinase C activator. Higher FN concentrations were less effective. The potentiation involved both initial rate and total CL responses and was more active on extracellular than intracellular generation of oxygen radicals. FN potentiation persisted after cell washing and was abolished by treatment of FN with trypsin. Measurement of the respiratory burst using the cytochrome c reduction assay confirmed that FN enhanced both the initial rate and total amount of Superoxide anion generated by FMLP-stimulated PMNs. These data indicate that FN facilitates the respiratory burst of chemoattractant-stimulated PMNs and suggest that FN can prepare PMNs in suspension for amplified biological functions induced by soluble inflammatory stimuli. Plenum Publishing Corporation, 1990 Stanislawski Lena Laboratoire d'Hématologie INSERM U 294, Hopital Bichat, France aut Huu Trung Laboratoire d'Hématologie INSERM U 294, Hopital Bichat, France aut Perianin Axel Département de Pharmacologie CNRS URA 595, Hopital Cochin Port Royal, Paris, France aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 523-530 0360-3997 14:5<523 1990 14 10753 https://doi.org/10.1007/BF00914273 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00914273 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stanislawski Lena Laboratoire d'Hématologie INSERM U 294, Hopital Bichat, France aut NATIONALLICENCE 700 1- Huu Trung Laboratoire d'Hématologie INSERM U 294, Hopital Bichat, France aut NATIONALLICENCE 700 1- Perianin Axel Département de Pharmacologie CNRS URA 595, Hopital Cochin Port Royal, Paris, France aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/5(1990-10-01), 523-530 0360-3997 14:5<523 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer