caa a22 4500 465764487 CHVBK 20180323111910.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00917453 doi (NATIONALLICENCE)springer-10.1007/BF00917453 Colonic inflammation in the rabbit induced by phorbol-12-myristate-13-acetate [Elektronische Daten] [D. Fretland, D. Widomski, S. Levin, T. Gaginella] Neutrophil (PMNL) infiltration of inflamed colonic tissue is a prominent feature of human inflammatory bowel disease (IBD). Colitis was established in New Zealand white rabbits by the intrarectal instillation of 1.5 mg/kg (in 10 ml 20% ethanol) phorbol-12-myristate-13-acetate (PMA) and assessed by visual grading of colonic inflammation, levels of the neutrophil marker enzyme myeloperoxidase (MPO), and histological examination. After 24 h there was a significant (P<0.001) increase in MPO levels in the PMA-treated colons compared to ethanol control. There was also increased inflammation based on visual scoring. Histologically, PMA-treated colons were necrotic with focal ulceration, heavy PMNL infiltration and edema at 24 h; by 96 h colitis was sustained with mild edema, crypt abscesses, and a staining pattern suggesting altered mucus quality. These results suggest that PMA-induced colitis in rabbits may be a new model of IBD in which to evaluate drugs known to mitigate the inflammatory process. Plenum Publishing Corporation, 1990 Fretland D. Department of Gastrointestinal Diseases Research, G. D. Searle and Co., 4901 Searle Parkway, 60077, Skokie, Illinois aut Widomski D. Department of Gastrointestinal Diseases Research, G. D. Searle and Co., 4901 Searle Parkway, 60077, Skokie, Illinois aut Levin S. Department of Gastrointestinal Diseases Research, G. D. Searle and Co., 4901 Searle Parkway, 60077, Skokie, Illinois aut Gaginella T. Department of Gastrointestinal Diseases Research, G. D. Searle and Co., 4901 Searle Parkway, 60077, Skokie, Illinois aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/2(1990-04-01), 143-150 0360-3997 14:2<143 1990 14 10753 https://doi.org/10.1007/BF00917453 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00917453 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fretland D. Department of Gastrointestinal Diseases Research, G. D. Searle and Co., 4901 Searle Parkway, 60077, Skokie, Illinois aut NATIONALLICENCE 700 1- Widomski D. Department of Gastrointestinal Diseases Research, G. D. Searle and Co., 4901 Searle Parkway, 60077, Skokie, Illinois aut NATIONALLICENCE 700 1- Levin S. Department of Gastrointestinal Diseases Research, G. D. Searle and Co., 4901 Searle Parkway, 60077, Skokie, Illinois aut NATIONALLICENCE 700 1- Gaginella T. Department of Gastrointestinal Diseases Research, G. D. Searle and Co., 4901 Searle Parkway, 60077, Skokie, Illinois aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/2(1990-04-01), 143-150 0360-3997 14:2<143 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer