caa a22 4500 465764517 CHVBK 20180323111910.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00917452 doi (NATIONALLICENCE)springer-10.1007/BF00917452 Con A-nonreactive human α1-acid glycoprotein (AGP) is more effective in modulation of lymphocyte proliferation than Con A-reactive AGP serum variants [Elektronische Daten] [Odette Pos, Robert Oostendorp, Marleen Van Der Stelt, Rik Scheper, Willem Van Dijk] Humanα 1-acid glycoprotein (AGP) has been shown to modulate various cellular and humoral immune reactions in vitro. Using glycosidase-modified derivatives of AGP, the importance of its carbohydrate moiety with regard to these effects has been noted. In normal serum, three molecular AGP forms interacting differently with concanavalin A (Con A) are present. The ratio of these forms is often changed during various physiopathological conditions. In this study, we could show that differences exist between the three AGP forms with regard to their immunomodulatory effectiveness. At physiological concentrations, the Con A-nonreactive variant AGP-A induced a stronger inhibition of the anti-CD3 stimulated lymphocyte proliferation than the other forms. Interestingly, AGP-A was also found to be responsible for the stimulation of lymphocyte proliferation induced by low AGP concentrations in vitro. Both immunomodulatory effects of AGP were abrogated by desialylation of the glycoprotein. These results support an immunomodulatory role of AGP in conditions characterized by a changed ratio of the differently glycosylated AGP forms. Plenum Publishing Corporation, 1990 Pos Odette Department of Medical Chemistry, Faculty of Medicine, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut Oostendorp Robert Department of Pathology and Immunology, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut Van Der Stelt Marleen Department of Medical Chemistry, Faculty of Medicine, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut Scheper Rik Department of Pathology and Immunology, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut Van Dijk Willem Department of Medical Chemistry, Faculty of Medicine, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut Inflammation Kluwer Academic Publishers-Plenum Publishers 14/2(1990-04-01), 133-141 0360-3997 14:2<133 1990 14 10753 https://doi.org/10.1007/BF00917452 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00917452 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pos Odette Department of Medical Chemistry, Faculty of Medicine, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Oostendorp Robert Department of Pathology and Immunology, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Van Der Stelt Marleen Department of Medical Chemistry, Faculty of Medicine, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Scheper Rik Department of Pathology and Immunology, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Van Dijk Willem Department of Medical Chemistry, Faculty of Medicine, Free University, P.O. Box 7161, 1007, MC Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Inflammation Kluwer Academic Publishers-Plenum Publishers 14/2(1990-04-01), 133-141 0360-3997 14:2<133 1990 14 10753 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer