caa a22 4500 465764827 CHVBK 20180323111910.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00155594 doi (NATIONALLICENCE)springer-10.1007/BF00155594 Genetic recombination in human melanoma and astrocytoma cell lines involves oncogenes and growth factor genes [Elektronische Daten] [M. Lakshmi, G. Sherbet] We describe here the chromosomal distribution of sister chromatid exchanges (SCEs) in four human tumor cell lines (two melanomas and two astrocytomas), and have mapped the sister chromatid recombination (SCR) sites. A higher incidence of SCR sites than expected on the basis of chromosome length occurred in chromosomes 2, 4, 5 and 15 in both the RPMI 5966 and MEL57 melanoma cell lines, and in chromosomes 1, 5, 13 and 15 of the IJKt and GUVW astrocytoma cell lines. A majority of the recombination sites occurred close to chromosomal fragile sites. A third of these occurred at the same bands as fragile sites. The recombination sites involved the N-ras and the epidermal growth factor gene in the melanomas. In the astrocytomas, the N-ras, Rb and c-mos genes appeared to be involved in the recombination events. The β2-microglobulin gene was involved in both astrocytomas and one melanoma. The erb132 was involved in SCR only in the RPMI melanoma. Taylor & Francis Ltd, 1990 Lakshmi M. Cancer Research Unit, Division of Oncology, University of Newcastle upon Tyne, The Medical School, NE2 4HH, Newcastle upon Tyne, U.K. aut Sherbet G. Cancer Research Unit, Division of Oncology, University of Newcastle upon Tyne, The Medical School, NE2 4HH, Newcastle upon Tyne, U.K. aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/1(1990-01-01), 75-87 0262-0898 8:1<75 1990 8 10585 https://doi.org/10.1007/BF00155594 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00155594 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lakshmi M. Cancer Research Unit, Division of Oncology, University of Newcastle upon Tyne, The Medical School, NE2 4HH, Newcastle upon Tyne, U.K aut NATIONALLICENCE 700 1- Sherbet G. Cancer Research Unit, Division of Oncology, University of Newcastle upon Tyne, The Medical School, NE2 4HH, Newcastle upon Tyne, U.K aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/1(1990-01-01), 75-87 0262-0898 8:1<75 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer