caa a22 4500 465764843 CHVBK 20180323111910.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00155592 doi (NATIONALLICENCE)springer-10.1007/BF00155592 Expression of a specific protein in spontaneously metastatic fibrosarcoma cell lines and its enhanced synthesis by growth on laminin or fibronectin [Elektronische Daten] [J. Youngblom, N. Wang, J. McCarthy, M. Janatipour, T. Wang, J. Sheppard, S. Panter] A panel of tumor cell lines and clones was generated by selecting for different metastatic capacity in 3AM fibrosarcoma cells. These cell lines were exposed to substrata of various purified extracellular matrix (ECM) proteins and labeled in culture with [35S]methionine. Following analysis by two-dimensional polyacrylamide gel electrophoresis the profiles of total cellular proteins produced by the cell lines displaying different phenotypes were examined. The presence of a specific protein, p54, was related to the cellular metastatic potential, and the synthesis of p54 was influenced by growth on extracellular matrix components. The amount of p54 was minimal and non-inducible in tumor cell lines exhibiting two different phenotypes: (1) experimentally metastatic (EM) and (2) transformed, non-tumorigenic (NTT) cell types. In contrast, all of the five different cell lines capable of both spontaneous and experimental metastasis (SEM), produced p54 either constitutively or through induction by growth on ECM protein substrata of either laminin of fibronectin, but not collagen type IV. These data suggest that the p54 protein may be a unique biochemical marker associated with spontaneous metastatic cell types. Taylor & Francis Ltd, 1990 Youngblom J. Department of Genetics and Cell Biology, University of Minnesota, 55455, Minneapolis, MN aut Wang N. Departments of Pediatrics, Genetics, and Pathology, University of Rochester School of Medicine, 14642, Rochester, NY, U.S.A. aut McCarthy J. Department of Laboratory Medicine and Pathology, University of Minnesota, 55455, Minneapolis, MN, U.S.A. aut Janatipour M. Department of Laboratory Medicine and Pathology, University of Minnesota, 55455, Minneapolis, MN, U.S.A. aut Wang T. Department of Surgery, University of Rochester School of Medicine, 14642, Rochester, NY, U.S.A. aut Sheppard J. Dight Laboratories, University of Minnesota, 55455, Minneapolis, MN aut Panter S. Department of Laboratory Medicine and Pathology, University of Minnesota, 55455, Minneapolis, MN, U.S.A. aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/1(1990-01-01), 47-58 0262-0898 8:1<47 1990 8 10585 https://doi.org/10.1007/BF00155592 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00155592 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Youngblom J. Department of Genetics and Cell Biology, University of Minnesota, 55455, Minneapolis, MN aut NATIONALLICENCE 700 1- Wang N. Departments of Pediatrics, Genetics, and Pathology, University of Rochester School of Medicine, 14642, Rochester, NY, U.S.A aut NATIONALLICENCE 700 1- McCarthy J. Department of Laboratory Medicine and Pathology, University of Minnesota, 55455, Minneapolis, MN, U.S.A aut NATIONALLICENCE 700 1- Janatipour M. Department of Laboratory Medicine and Pathology, University of Minnesota, 55455, Minneapolis, MN, U.S.A aut NATIONALLICENCE 700 1- Wang T. Department of Surgery, University of Rochester School of Medicine, 14642, Rochester, NY, U.S.A aut NATIONALLICENCE 700 1- Sheppard J. Dight Laboratories, University of Minnesota, 55455, Minneapolis, MN aut NATIONALLICENCE 700 1- Panter S. Department of Laboratory Medicine and Pathology, University of Minnesota, 55455, Minneapolis, MN, U.S.A aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/1(1990-01-01), 47-58 0262-0898 8:1<47 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer