caa a22 4500 465764886 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00155590 doi (NATIONALLICENCE)springer-10.1007/BF00155590 Interleukin-1 increases tumor cell adhesion to endothelial cells through an RGD dependent mechanism: in vitro and in vivo studies [Elektronische Daten] [Davide Lauri, Maria-Cruz Bertomeu, F. Orr, Eva Bastida, D. Sauder, Michael Buchanan] The effects of human recombinant interleukin-1α and β (rIL-1α; rIL-1β) on the adhesion of human A549 lung carcinoma cells and M6 melanoma cells (TC) to human endothelial cells (HECs) in vitro were studied, and on TC/lung entrapment in vivo. In vitro, there was a significant increase in TC/HEC adhesion to HECs pretreated for 4 h with rIL-1α or rIL-1β. The effects of rIL-1α and β on TC/HEC adhesion were time dependent and reached a plateau within 4-6h. TC/HEC adhesion was not blocked when measured in the presence of antibodies to either fibronectin, glycoprotein IIb/IIIa, anti-ICAM, or anti-LFA. However, enhanced TC/HEC adhesion was completely blocked in the presence of the peptide, GRGDS. In vivo, pretreatment of nude mice for 4 h with rIL-1α (given i.p. before ix. injection of TCs) enhanced TC retention in the lung 24 h later. Our data demonstrate that IL-1 enhances TC adhesion to the vascular surface both in vitro and in vivo, suggesting that IL-1 can facilitate the metastatic process. Taylor & Francis Ltd, 1990 Lauri Davide Departments of Pathology and Medicine, McMaster University, Hamilton, Ontario, Canada aut Bertomeu Maria-Cruz Departments of Pathology and Medicine, McMaster University, Hamilton, Ontario, Canada aut Orr F. Istituto Mario Negri, Milano, Italy aut Bastida Eva Hospital 10585ic i Provincial, Barcelona, Spain aut Sauder D. Departments of Pathology and Medicine, McMaster University, Hamilton, Ontario, Canada aut Buchanan Michael Departments of Pathology and Medicine, McMaster University, Hamilton, Ontario, Canada aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/1(1990-01-01), 27-32 0262-0898 8:1<27 1990 8 10585 https://doi.org/10.1007/BF00155590 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00155590 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lauri Davide Departments of Pathology and Medicine, McMaster University, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Bertomeu Maria-Cruz Departments of Pathology and Medicine, McMaster University, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Orr F. Istituto Mario Negri, Milano, Italy aut NATIONALLICENCE 700 1- Bastida Eva Hospital 10585ic i Provincial, Barcelona, Spain aut NATIONALLICENCE 700 1- Sauder D. Departments of Pathology and Medicine, McMaster University, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Buchanan Michael Departments of Pathology and Medicine, McMaster University, Hamilton, Ontario, Canada aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/1(1990-01-01), 27-32 0262-0898 8:1<27 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer