caa a22 4500 465764940 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00135873 doi (NATIONALLICENCE)springer-10.1007/BF00135873 In vitro effects of bone- and platelet-derived transforming growth factor-β on the growth of Walker 256 carcinosarcoma cells [Elektronische Daten] [W. Millar-Book, F. Orr, G. Singh] Conditioned media from fetal rat calvarial cultures has previously been shown to stimulate the growth of the bone-metastasizing Walker 256 carcinosarcoma cell line. In the current investigation we looked at the possibility that transforming growth factor-β (TGF-β), present in conditioned media, and positively correlated with resorption in vitro, may be responsible for the enhanced proliferation of Walker cells cultured in these conditioned media. Purified platelet-derived TGF-β produced a dose-dependent growth response in Walker cells with an ED50 equal to 0·05 ng/ml. Bone-derived TGF-β activity in conditioned media, measured by NRK fibroblast colony formation, correlated well with percentage resorption in bone cultures, and growth activity in Walker cell culture. In addition to this, the growth response normally seen with conditioned media cultures of Walker cells was significantly inhibited by the addition of anti-TGF-β1 neutralizing antibody. We conclude that TGF-β is an important growth stimulatory component from fetal rat calvaria. Taylor & Francis Ltd, 1990 Millar-Book W. Department of Pathology and Oncology Research Group, McMaster University, Hamilton, Ontario, Canada aut Orr F. Department of Pathology and Oncology Research Group, McMaster University, Hamilton, Ontario, Canada aut Singh G. Department of Pathology and Oncology Research Group, McMaster University, Hamilton, Ontario, Canada aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/6(1990-11-01), 503-510 0262-0898 8:6<503 1990 8 10585 https://doi.org/10.1007/BF00135873 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00135873 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Millar-Book W. Department of Pathology and Oncology Research Group, McMaster University, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Orr F. Department of Pathology and Oncology Research Group, McMaster University, Hamilton, Ontario, Canada aut NATIONALLICENCE 700 1- Singh G. Department of Pathology and Oncology Research Group, McMaster University, Hamilton, Ontario, Canada aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/6(1990-11-01), 503-510 0262-0898 8:6<503 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer