caa a22 4500 465764959 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00135878 doi (NATIONALLICENCE)springer-10.1007/BF00135878 Induction of invasive and metastatic potential in mouse T-lymphoma cells (BW5147) by treatment with 5-azacytidine [Elektronische Daten] [G. Habets, R. Van Der Kammen, E. Scholtes, J. Collard] Non-invasive, non-metastatic mouse BW5147 T-lymphoma cells were treated with non-mutagenic concentrations of the hypomethylating agent 5-azacytidine (5-aza-C). Subsequently, invasive variants were selected on monolayers of rat embryo fibroblasts. The estimated frequency of induction of invasive variants was smaller than 1 in 106 cells. We obtained several independent clones that were stable in the expression of the invasive phenotype. In contrast to the parental cell line, the highly invasive clones produced widespread metastases upon tail vein injection in all the syngeneic AKR mice tested, whereas' clones with an intermediate level of invasiveness formed metastases only in part of the mice tested. DNA analysis using the methylation-sensitive and insensitive restriction enzymes, Hpa-II and Msp-I, respectively, showed that the DNA of the invasive variants remained hypomethylated, up to 6 months after 5-aza-C treatment. 5-aza-C is thus able to induce invasive and metastatic potential in the BW5147 T-lymphoma cells, similar to the activated human c-Ha-ras oncogene or human chromosome 7, as studied previously. The acquisition of invasive and metastatic potential is presumably caused by DNA hypomethylation and thus activation of one or more silent invasion controlling genes. Taylor & Francis Ltd, 1990 Habets G. Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066, CX Amsterdam, The Netherlands aut Van Der Kammen R. Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066, CX Amsterdam, The Netherlands aut Scholtes E. Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066, CX Amsterdam, The Netherlands aut Collard J. Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066, CX Amsterdam, The Netherlands aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/6(1990-11-01), 567-577 0262-0898 8:6<567 1990 8 10585 https://doi.org/10.1007/BF00135878 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00135878 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Habets G. Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066, CX Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Van Der Kammen R. Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066, CX Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Scholtes E. Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066, CX Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Collard J. Division of Cell Biology, The Netherlands Cancer Institute, 121 Plesmanlaan, 1066, CX Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/6(1990-11-01), 567-577 0262-0898 8:6<567 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer