caa a22 4500 465764967 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00141257 doi (NATIONALLICENCE)springer-10.1007/BF00141257 Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis [Elektronische Daten] [J. Williams III, M. Smith, D. Carlson, C. Williams, K. Tweedell, R. McKinnell] PNKT-4B is an aneuploid cell line derived from a herpesvirus-induced renal adenocarcinoma of Rana pipiens that displays restricted invasion at 21°C or cooler and invasion at 23°C through 28°C. Metaphase chromosomes obtained from subcultures (passages 297; 345-347) grown at 18°C or 28°C were Giemsa stained or N-banded with acidic silver nitrate. Cells grown at 18°C displayed a modal chromosome number of 41, while 28°C cultures displayed a modal number of 40. The distribution of the chromosomes suggests that the two temperatures may be allowing growth of different subclonal populations. N-banding of chromosomes at both temperatures revealed an increase of active nucleolar organizer regions (NORs) over normal frog tissues, 2/2N. Analysis of 200 N-banded spreads from cells grown at each temperature revealed modal numbers of 9 NORs/cell and modal numbers of 6 NOR-containing chromosomes/cell. Nine specific NOR-containing chromosomes were identified and scored. Similar distributions were observed at 18°C and 28°C. The data imply that the modal number of PNKT-4B has shifted since it was first described, 39, and differs at invasion-permissive and — restrictive temperatures. Increased numbers of active NORs and alterations of NOR-containing chromosomes imply an amplification of rDNA over the amount in normal frog. Taylor & Francis Ltd., 1990 Williams III J. Department of Biology, Tuskegee University, 36088, Tuskegee, Alabama, USA aut Smith M. Department of Biology, Tuskegee University, 36088, Tuskegee, Alabama, USA aut Carlson D. Department of Genetics and Cell Biology, University of Minnesota, 55108-1095, St Paul, Minnesota, USA aut Williams C. Department of Biology, Tuskegee University, 36088, Tuskegee, Alabama, USA aut Tweedell K. Department of Biological Science, University of Notre Dame, 46556, Notre Dame, Indiana, U.S.A. aut McKinnell R. Department of Genetics and Cell Biology, University of Minnesota, 55108-1095, St Paul, Minnesota, USA aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 267-276 0262-0898 8:3<267 1990 8 10585 https://doi.org/10.1007/BF00141257 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00141257 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Williams III J. Department of Biology, Tuskegee University, 36088, Tuskegee, Alabama, USA aut NATIONALLICENCE 700 1- Smith M. Department of Biology, Tuskegee University, 36088, Tuskegee, Alabama, USA aut NATIONALLICENCE 700 1- Carlson D. Department of Genetics and Cell Biology, University of Minnesota, 55108-1095, St Paul, Minnesota, USA aut NATIONALLICENCE 700 1- Williams C. Department of Biology, Tuskegee University, 36088, Tuskegee, Alabama, USA aut NATIONALLICENCE 700 1- Tweedell K. Department of Biological Science, University of Notre Dame, 46556, Notre Dame, Indiana, U.S.A aut NATIONALLICENCE 700 1- McKinnell R. Department of Genetics and Cell Biology, University of Minnesota, 55108-1095, St Paul, Minnesota, USA aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 267-276 0262-0898 8:3<267 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer