caa a22 4500 465764975 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00141255 doi (NATIONALLICENCE)springer-10.1007/BF00141255 Altered glycosylation in Madin-Darby canine kidney (MDCK) cells after transformation by murine sarcoma virus [Elektronische Daten] [E. Bruyneel, H. Hebray, M. De Mets, M. Mareel, J. Montreuil] The changes in glycosylation of an immortalized epithelial cell line (MDCK) before and after progression towards a more malignant phenotype have been studied. The parental MDCK-3 cells were immortalized after long-term passage in vitro and have shown no tendency for spontaneous acquisition of malignancy-related phenotypes such as tumorigenicity, invasion and metastasis. They conserved morphological and functional characteristics of the epithelial tissue of origin. The ras-MDCK cells acquired the fully malignant phenotype after transformation with a Harvey murine sarcoma virus; they were immortalized, invasive in vitro and produced invasive and also metastatic tumors after subcutaneous injection into nude mice. Using immobilized lectins and gel chromatography, before and after liberation of O-linked glycans from the peptide moieties and also after removal of terminal sialic acid, we have found differences in the glycosylpeptides of both whole cells and cell surface trypsinates from ras-MDCK cultures as compared to the parental MDCK-3 cultures: (i) more sialic acid in the N-linked tri- and tetra-antennary structures; (ii) more fucosylation in the N-glycosylpeptides; (iii) more bi-antennary N-glycosylpeptides and less O-linked glycans; and (iv) a lower molecular weight of the O-linked glycans probably due to a decreased sialylation. It is concluded that alterations in sialylation and fucosylation of the cell surface exposed glycans accompanied progression of MDCK-3 cells towards a more malignant phenotype. Taylor & Francis Ltd., 1990 Bruyneel E. Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, University Hospital, De Pintelaan 185, B-9000, Ghent, Belgium aut Hebray H. Unite Mixte de Recherche du CNRS no. 111, Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, F-59655, Villeneuve d'Ascq Cédex, France aut De Mets M. Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, University Hospital, De Pintelaan 185, B-9000, Ghent, Belgium aut Mareel M. Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, University Hospital, De Pintelaan 185, B-9000, Ghent, Belgium aut Montreuil J. Unite Mixte de Recherche du CNRS no. 111, Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, F-59655, Villeneuve d'Ascq Cédex, France aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 241-253 0262-0898 8:3<241 1990 8 10585 https://doi.org/10.1007/BF00141255 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00141255 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bruyneel E. Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, University Hospital, De Pintelaan 185, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Hebray H. Unite Mixte de Recherche du CNRS no. 111, Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, F-59655, Villeneuve d'Ascq Cédex, France aut NATIONALLICENCE 700 1- De Mets M. Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, University Hospital, De Pintelaan 185, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Mareel M. Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, University Hospital, De Pintelaan 185, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Montreuil J. Unite Mixte de Recherche du CNRS no. 111, Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, F-59655, Villeneuve d'Ascq Cédex, France aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 241-253 0262-0898 8:3<241 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer