caa a22 4500 465764983 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00141254 doi (NATIONALLICENCE)springer-10.1007/BF00141254 Tweedell Kenyon Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, U.S.A. aut Adhesion of frog pronephric tumor cells to normal cells cultivated on microcarrier beads [Elektronische Daten] [Kenyon Tweedell] The processes of cell adhesion and active spreading were assessed between frog normal pronephric, pronephric tumor and heterologous liver cells. Confluent monolayers were developed on collagen-coated microcarrier beads, then exposed to homologous or heterologous cells and cultivated with a rotary (orbital) flask culture technique at 23°C. All three cell lines attach and actively spread on the collagen-coated microcarrier beads. Secondary attachment of normal (non-transformed) proliferating cells to their confluent monolayers occurs but cell spreading is restrained. Dissociated pronephric tumor cells adhere and actively spread on the surfaces of normal pronephric cells, and eventually encapsulate them. Normal pronephric cells do not adhere readily to the cell surfaces of pronephric tumor cells grown on microcarrier beads. Tumor cells also attach, actively spread and overgrow heterologous liver cells attached to microcarrier beads. Suboptimal temperatures (17°C) slow tumor cell attachment and spreading on normal cells. Colder temperature (8°C) permits tumor cell attachment and adhesion to normal cell-coated beads but active cell spreading is prohibited. The same temperature retards cell spreading directly on the collagen-coated beads. Taylor & Francis Ltd., 1990 Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 227-239 0262-0898 8:3<227 1990 8 10585 https://doi.org/10.1007/BF00141254 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00141254 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Tweedell Kenyon Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, U.S.A aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 227-239 0262-0898 8:3<227 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer