caa a22 4500 465764991 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00141259 doi (NATIONALLICENCE)springer-10.1007/BF00141259 Lectin-resistant variants of mouse Lewis lung carcinoma cells. II. Altered glycosylation of membrane glycoproteins [Elektronische Daten] [H. Debray, D. Dus, P. Hueso, C. Radzikowski, J. Montreuil] Lectin-resistant variants of mouse Lewis lung carcinoma LL2 cell line, selected with wheat germ agglutinin (WGAR), Ricinus communis agglutinin II (RCA IIR) and Aleuria aurantia agglutinin (AAAR) were studied. Total cellular glycopeptides of the parent LL2 line and of the five lectin-resistant variants were analyzed by gel filtration and affinity chromatography on immobilized concanavalin A and Lens culinaris agglutinin. The results revealed that low-metastatic WGAR and RCA IIR variants possessed less highly branched tri- and tetra-antennary N-acetyllactosaminic type glycans with a simultaneous increase in biantennary N-acetyllactosaminic type, oligomannosidic type or hybrid type glycans, as compared to the parent metastasizing LL2 cell line. These findings imply that cell surface carbohydrate changes may possibly be relevant for metastasis. However, the AAAR variant, which possessed reduced spontaneous metastatic ability after s.c. administration, but increased experimental metastatic ability after i.v. inoculation, exhibited apparently the same glycan pattern than the parent LL2 line. This particular variant is under investigation in order to find specific modification(s) of glycan(s) which could play a specific role in the metastatic process. Taylor & Francis Ltd., 1990 Debray H. Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois (Unité Mixte de Recherche CNRS no. 111), 59655, Villeneuve d'Ascq Cedex, France aut Dus D. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wrocław, Poland aut Hueso P. Faculty of Veterinary Sciences, Department of Biochemistry and Molecular Biology, University of Leon, 24007, Leon, Spain aut Radzikowski C. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wrocław, Poland aut Montreuil J. Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois (Unité Mixte de Recherche CNRS no. 111), 59655, Villeneuve d'Ascq Cedex, France aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 287-298 0262-0898 8:3<287 1990 8 10585 https://doi.org/10.1007/BF00141259 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00141259 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Debray H. Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois (Unité Mixte de Recherche CNRS no. 111), 59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Dus D. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wrocław, Poland aut NATIONALLICENCE 700 1- Hueso P. Faculty of Veterinary Sciences, Department of Biochemistry and Molecular Biology, University of Leon, 24007, Leon, Spain aut NATIONALLICENCE 700 1- Radzikowski C. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wrocław, Poland aut NATIONALLICENCE 700 1- Montreuil J. Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois (Unité Mixte de Recherche CNRS no. 111), 59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 287-298 0262-0898 8:3<287 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer