caa a22 4500 465765009 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00141256 doi (NATIONALLICENCE)springer-10.1007/BF00141256 Differences in organization of metastatic and nonmetastatic tumors initiated by the same B16 melanoma clone in mature and young mice [Elektronische Daten] [C. Stackpole, A. Alterman, C. Angadi, Y. Kim, D. Fornabaio] Subcutaneous transplants of mouse B16 melanoma clone G3.26 grow more slowly, and are markedly more metastatic to the lungs, in mature (> 12-monthold) mice than in young (2-month-old) mice. Previous studies suggested that tumors in young mice fail to disseminate viable tumor cells into the hematogenous circulation. To determine if changes in intratumor organization might accompany this altered tumor behavior, G3.26 tumors growing in young and mature mice were examined comparatively at progressive sizes relative to the onset of metastatic dissemination in the older mice. Although the degree of necrosis was comparable in both groups of tumors, vascular density, measured morphometrically in histological sections, was significantly lower in tumors from mature mice at a size when dissemination would be occurring. With the onset of reduced vascular density in tumors in mature mice, there was a substantial increase in the proportion of viable tumor cells that was hypoxic, based on radioresistance and incorporation of the hypoxic cell sensitizer, misonidazole. Quiescent tumor cells, identified by flow cytometry, were also more numerous in tumors from mature mice than in tumors from young mice. Although the importance of these differences in tumor organization to enhanced metastatic behavior is unclear, increased intratumor hypoxia might promote generation of metastatic variants. Alternately, dissemination of tumor cells might be facilitated through a reduced and possibly defective vasculature. Taylor & Francis Ltd., 1990 Stackpole C. Department of Pathology, New York Medical College, 10595, Valhalla, New York, U.S.A. aut Alterman A. Department of Pathology, New York Medical College, 10595, Valhalla, New York, U.S.A. aut Angadi C. Department of Pathology, Nassau County Medical Center, 11554, East Meadow, New York, U.S.A. aut Kim Y. Department of Pathology, New York Medical College, 10595, Valhalla, New York, U.S.A. aut Fornabaio D. Department of Pathology, New York Medical College, 10595, Valhalla, New York, U.S.A. aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 255-266 0262-0898 8:3<255 1990 8 10585 https://doi.org/10.1007/BF00141256 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00141256 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stackpole C. Department of Pathology, New York Medical College, 10595, Valhalla, New York, U.S.A aut NATIONALLICENCE 700 1- Alterman A. Department of Pathology, New York Medical College, 10595, Valhalla, New York, U.S.A aut NATIONALLICENCE 700 1- Angadi C. Department of Pathology, Nassau County Medical Center, 11554, East Meadow, New York, U.S.A aut NATIONALLICENCE 700 1- Kim Y. Department of Pathology, New York Medical College, 10595, Valhalla, New York, U.S.A aut NATIONALLICENCE 700 1- Fornabaio D. Department of Pathology, New York Medical College, 10595, Valhalla, New York, U.S.A aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 255-266 0262-0898 8:3<255 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer