caa a22 4500 465765017 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00141258 doi (NATIONALLICENCE)springer-10.1007/BF00141258 Lectin-resistant variants of mouse Lewis lung carcinoma cells. I. Selection and in vivo properties [Elektronische Daten] [D. Dus, H. Debray, L. strzadala, J. Rak, H. Kusnierczyk, J. Montreuil, C. Radzikowski] The availability of lectin-resistant cell lines with altered carbohydrate moieties in cell surface glycoproteins and glycolipids has greatly facilitated study of the involvement of cellular glycoconjugates in tumor growth and metastasis. We present here a new animal model for metastasis study based on mouse Lewis lung carcinoma LL2 in vitro cell line [10]. From this line, five lectin-resistant variant sublines were selected with the following lectins: wheat germ agglutinin (WGAR), Ricinus communis agglutinin II (RCA IIR) and Aleuria aurantia agglutinin (AAAR). The correlation of the lectin resistance with their in vitro and in vivo growth properties, and especially lung colonizing ability, were investigated. Three WGAR variants with well-preserved tumorigenicity revealed reduced metastatic ability, both spontaneous, after subcutaneous (s.c.) administration and experimental, after intravenous (i.v.) administration. The RCA IIR variant also possessed reduced spontaneous and experimental metastatic ability, but exhibited higher growth rate of local s.c. tumors. The AAAR variant possessed reduced spontaneous metastatic ability but its ability to colonize the lungs after i.v. administration was five-fold higher than that of the parent LLZ line, whereas its tumorigenicity remained unchanged. The relative differences among WGAR variants and parent LLZ line, concerning their experimental metastatic ability, remained similar in cyclophosphamide-modified mice to those in normal recipients. Taylor & Francis Ltd., 1990 Dus D. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut Debray H. Laboratoire de Chimie Biologique de I'Université des Sciences et Techniques de Lille Flandres-Artois (Unite Mixte de Recherche MRS no. 111), 59655 Villeneuve d'Ascq Cedex, France aut strzadala L. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut Rak J. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut Kusnierczyk H. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut Montreuil J. Laboratoire de Chimie Biologique de I'Université des Sciences et Techniques de Lille Flandres-Artois (Unite Mixte de Recherche MRS no. 111), 59655 Villeneuve d'Ascq Cedex, France aut Radzikowski C. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 277-286 0262-0898 8:3<277 1990 8 10585 https://doi.org/10.1007/BF00141258 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00141258 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dus D. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut NATIONALLICENCE 700 1- Debray H. Laboratoire de Chimie Biologique de I'Université des Sciences et Techniques de Lille Flandres-Artois (Unite Mixte de Recherche MRS no. 111), 59655 Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- strzadala L. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut NATIONALLICENCE 700 1- Rak J. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut NATIONALLICENCE 700 1- Kusnierczyk H. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut NATIONALLICENCE 700 1- Montreuil J. Laboratoire de Chimie Biologique de I'Université des Sciences et Techniques de Lille Flandres-Artois (Unite Mixte de Recherche MRS no. 111), 59655 Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Radzikowski C. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114, Wroclaw, Poland aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/3(1990-05-01), 277-286 0262-0898 8:3<277 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer