caa a22 4500 465765076 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01810677 doi (NATIONALLICENCE)springer-10.1007/BF01810677 Basement membrane invasion by first trimester human trophoblast: requirement for branched complex-type Asn-linked oligosaccharides [Elektronische Daten] [S. Yagel, R. Kerbel, P. Lala, T. Eldar-Gera, J. Dennis] Trophoblast cells of normal first trimester human placenta share with malignant tumor cells the ability for significant cellular proliferation and invasion of basement membranes. Because tumor cell metastasisin vivo and invasion of basement membranesin vitro have recently been shown to require the expression of-GIcNAcβ1-6 Manαl-6 Manβl-branched complex type Asn-linked oligosaccharides in tumor cell surface glycoproteins, we decided to determine if such structures were also necessary for invasion by trophoblast cells. We report here that invasive first trimester trophoblasts express leukoagglutinin-reactive βl-6 branched Asn-linked oligosaccharides on their surface. Moreover, basement membrane invasion by trophoblast was significantly inhibited by pretreating the cells with swainsonine, a non-toxic inhibitor of Golgi α-mannosidase II which blocks β1-6 branching of Asn-linked oligosaccharides. The first trimester trophoblast cells pretreated with swainsonine attached more avidly to the amnion basement membrane and to an extracellular matrix (ECM) preparation compared to control non-treated erophoblast cells. Swainsonine treatment did not inhibit secretion of gelatinase or plasminogen activator activities by trophoblast cells. These results suggest that expression of βl-6 branched oligosaccharides in trophoblast cells may be functionally important for the implantation and placentation processes by reducing cell adhesion to ECM and thereby facilitating trophoblast cell invasion. Taylor & Francis Ltd., 1990 Yagel S. Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, 600 University Avenue, M5G 1X5, Toronto, Ontario aut Kerbel R. Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, 600 University Avenue, M5G 1X5, Toronto, Ontario aut Lala P. Department of Anatomy, The University of Western Ontario, N6A 5C1, London, Ontario, Canada aut Eldar-Gera T. The Department of Obstetrics and Gynecology, Hadassah University Hospital, Jerusalem, Israel aut Dennis J. Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, 600 University Avenue, M5G 1X5, Toronto, Ontario aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/4(1990-07-01), 305-317 0262-0898 8:4<305 1990 8 10585 https://doi.org/10.1007/BF01810677 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01810677 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yagel S. Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, 600 University Avenue, M5G 1X5, Toronto, Ontario aut NATIONALLICENCE 700 1- Kerbel R. Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, 600 University Avenue, M5G 1X5, Toronto, Ontario aut NATIONALLICENCE 700 1- Lala P. Department of Anatomy, The University of Western Ontario, N6A 5C1, London, Ontario, Canada aut NATIONALLICENCE 700 1- Eldar-Gera T. The Department of Obstetrics and Gynecology, Hadassah University Hospital, Jerusalem, Israel aut NATIONALLICENCE 700 1- Dennis J. Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, 600 University Avenue, M5G 1X5, Toronto, Ontario aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/4(1990-07-01), 305-317 0262-0898 8:4<305 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer