caa a22 4500 465765106 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01810681 doi (NATIONALLICENCE)springer-10.1007/BF01810681 Sulfated glycoconjugate determinants recognized by monoclonal antibody, SG-1, correlate with the experimental metastatic ability of KHT fibrosarcoma cells [Elektronische Daten] [J. Harris, D. Beaton] We have examined the binding and functional activity of monoclonal antibody (MAb) SG-1 that was raised by immunization against embryonal carcinoma cells and screened using KHT fibrosarcoma cells. Quantitative absorption, binding andin situ immunochemical staining assays indicate that the MAb SG-1-defined epitopes are expressed preferentially by the highly metastatic KHT35-Ll cells relative to the weakly metastatic, parental KHTp cells. Furthermore, there was a significant correlation (p<0-05) between the expression of MAb SG-1-defined antigen on the cells, following trypsin treatment, and their metastatic ability. Binding of MAb SG-1 to antigen was inhibited by specific sulfated polysaccharides including cerebroside sulfate (brain sulfatide), fucoidan, and dextran sulfate (500 kD) but not by heparan, chrondroitin, keratan or dextran (5 kD) sulfates. Initial characterization of antigen from KHT cells indicates that the epitope of MAb SG-1 is defined by sulfated glycoconjugates containing galactose and sulfate but not N-acetylglucosamine. In the total lipid extracts of KHT35-Ll cells the antigen was detected in the delipidated protein fraction as well as in the chloroform/methanol fraction. These results suggest that the sulfated glycoconjugate determinants identified by MAb SG-1 may be relevant to the metastatic process of KHT fibrosarcoma cells. Taylor & Francis Ltd., 1990 Harris J. Departments of Oncology, Microbiology and Immunology, University of Western Ontario, and the London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut Beaton D. aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/4(1990-07-01), 361-379 0262-0898 8:4<361 1990 8 10585 https://doi.org/10.1007/BF01810681 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01810681 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harris J. Departments of Oncology, Microbiology and Immunology, University of Western Ontario, and the London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut NATIONALLICENCE 700 1- Beaton D. aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/4(1990-07-01), 361-379 0262-0898 8:4<361 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer