caa a22 4500 465765157 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00058157 doi (NATIONALLICENCE)springer-10.1007/BF00058157 The regulation of cyclic AMP production and the role of cyclic AMP in B16 melanoma cells of differing metastatic potential [Elektronische Daten] [S. Hill, R. Rees, S. MacNeil] : The nature of the relationship between agonist-stimulated cyclic AMP production and metastatic potential was examined in detail for four B16 melanoma cell lines of varying metastatic potential. Highly metastatic cells (B16 F10C1) appeared to differ from cells of low metastatic potential (B16 F1C29) in the degree to which cyclic AMP production in intact cells was stimulated by protein kinase C activation. No significant difference was found in the adenylate cyclase enzyme activities of the broken cells, irrespective of the agonist used, or in the distribution of cyclic AMP between the intracellular and extracellular compartment. Although B16 F1, F10 and F10C1 cells all produced equally pigmented tumors in vivo, the cells differed in their melanogenic response to cyclic AMP elevating agents in vitro: the least metastatic cells produced least agonist-induced cyclic AMP but this induced greatest tyrosinase activation and melanin production in vitro; conversely, the more metastatic cells produced more cyclic AMP but less tyrosinase activation and melanin production in response to agonist stimulation. Thus, agonist-stimulated cyclic AMP production does not appear to be coupled to the differentiated function of melanogenesis for highly metastatic B16 melanoma cells. Taylor & Francis Ltd, 1990 Hill S. Department of Medicine, Clinical Sciences Centre, Northern General Hospital, S5 7AU, Sheffield, U.K. aut Rees R. Department of Experimental and Clinical Microbiology, Section of Tumour Biology and Immunology, The Medical School, Beech Hill Road, S10 2RX, Sheffield, U.K. aut MacNeil S. Department of Medicine, Clinical Sciences Centre, Northern General Hospital, S5 7AU, Sheffield, U.K. aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/5(1990-09-01), 475-489 0262-0898 8:5<475 1990 8 10585 https://doi.org/10.1007/BF00058157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00058157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hill S. Department of Medicine, Clinical Sciences Centre, Northern General Hospital, S5 7AU, Sheffield, U.K aut NATIONALLICENCE 700 1- Rees R. Department of Experimental and Clinical Microbiology, Section of Tumour Biology and Immunology, The Medical School, Beech Hill Road, S10 2RX, Sheffield, U.K aut NATIONALLICENCE 700 1- MacNeil S. Department of Medicine, Clinical Sciences Centre, Northern General Hospital, S5 7AU, Sheffield, U.K aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/5(1990-09-01), 475-489 0262-0898 8:5<475 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer