caa a22 4500 465765181 CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00058153 doi (NATIONALLICENCE)springer-10.1007/BF00058153 ras Transfection and expression does not induce progression from tumorigenicity to metastatic ability in mouse LTA cells [Elektronische Daten] [A. Tuck, S. Wilson, A. Chambers] : Studies testing the ability of a transfected ras oncogene to confer metastatic properties on non-metastatic cells have yielded conflicting results. Most of these studies have used recipient cells at early stages of progression (primary or immortalized, non-tumorigenic lines). In this study we tested the ability of the T24-H-ras oncogene to induce progression of tumorigenic, non-metastatic, murine LTA cells to a metastatic phenotype. Metastatic ability was assessed in complementary assays in two immune-deficient hosts, nude mice (after s.c. injection) and chick embryos (after i.v. injection), to determine if ras transfection affected metastatic properties in hosts lacking an intact immune system. Even with greatly elevated levels of ras p21 protein, pools of ras-transfected cells as well as individual clonal populations remained non-metastatic in both hosts. Serial in vivo passaging did not consistently enhance for either ras expression or metastatic ability. We conclude that expression of an activated ras oncogene in LTA cells does not induce progression from a tumorigenic to a metastatic phenotype. These results are in marked contrast to those obtained for ras expression in most other cell types. High levels of expression of an activated ras oncogene thus do not always promote progression from tumorigenicity to metastatic ability. Taylor & Francis Ltd, 1990 Tuck A. London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut Wilson S. London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut Chambers A. London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/5(1990-09-01), 417-431 0262-0898 8:5<417 1990 8 10585 https://doi.org/10.1007/BF00058153 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00058153 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tuck A. London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut NATIONALLICENCE 700 1- Wilson S. London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut NATIONALLICENCE 700 1- Chambers A. London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/5(1990-09-01), 417-431 0262-0898 8:5<417 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer