caa a22 4500 46576522X CHVBK 20180323111911.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00117786 doi (NATIONALLICENCE)springer-10.1007/BF00117786 Hyaluronate-binding proteins in weakly and highly metastatic variants of rat rhabdomyosarcoma cells [Elektronische Daten] [Madeleine Moczar, Marie Poupon, Elemer Moczar] : Weakly (RMS8) and highly (RMS0) metastatic rat rhabdomyosarcoma cells were assayed for their interaction with hyaluronate. The cells in subconfluent cultures were incubated with 35S methionine, the cells were fractionated and the labelled proteins were separated by affinity chromatography on hyaluronate-Sepharose and by HPLC. The RMS8 cells expressed about twice the amount of labelled hyaluronate-binding proteins seen in the RMS0 cells. The molecular sizes of the main hyaluronate-binding proteins were similar in both cell types. Unlike the RMS0 cells, the RMS8 cells took up exogenous, radioactively labelled hyaluronate at 4°C in a saturable and specific way with high affinity. Cells were also incubated with 3H glucosamine. The isolation of the glycosaminoglycans from these cultures by ion-exchange chromatography indicated that the RMS8 cells retained more endogenous 3H hyaluronate in their pericellular domain than did the RMS0 cells. The attachment of trypsinized cells could be inhibited with exogenous hyaluronate, indicating that the proteins with affinity for hyaluronate may act as hyaluronate-binding sites on these cells. Taylor & Francis Ltd, 1990 Moczar Madeleine Laboratoire de Biochimie du Tissu Conjonctif, UA CNRS, 8 rue du General Sarrail, No1174, F-94010, Créteil, France aut Poupon Marie Laboratoire des Metastases, IRSC du CNRS, 7 rue Guy Mocquet, F-94800, Villejuif, France aut Moczar Elemer Laboratoire de Biochimie du Tissu Conjonctif, UA CNRS, 8 rue du General Sarrail, No1174, F-94010, Créteil, France aut Clinical & Experimental Metastasis Kluwer Academic Publishers 8/2(1990-03-01), 129-140 0262-0898 8:2<129 1990 8 10585 https://doi.org/10.1007/BF00117786 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00117786 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moczar Madeleine Laboratoire de Biochimie du Tissu Conjonctif, UA CNRS, 8 rue du General Sarrail, No1174, F-94010, Créteil, France aut NATIONALLICENCE 700 1- Poupon Marie Laboratoire des Metastases, IRSC du CNRS, 7 rue Guy Mocquet, F-94800, Villejuif, France aut NATIONALLICENCE 700 1- Moczar Elemer Laboratoire de Biochimie du Tissu Conjonctif, UA CNRS, 8 rue du General Sarrail, No1174, F-94010, Créteil, France aut NATIONALLICENCE 773 0- Clinical & Experimental Metastasis Kluwer Academic Publishers 8/2(1990-03-01), 129-140 0262-0898 8:2<129 1990 8 10585 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer