caa a22 4500 465765394 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02562421 doi (NATIONALLICENCE)springer-10.1007/BF02562421 Intestinal absorption of unconjugated dihydroxy bile acids: Non-mediation by the carrier system involved in long chain fatty acid absorption [Elektronische Daten] [Wolfgang Stremmel, Alan Hofmann] Experiments were performed using isolated mucosal cells from the rat jejunum or using the perfused jejunum in the anesthetized rat to test whether lipophilic unconjugated dihydroxy bile acids are absorbed from the proximal small intestine via the same carrier mechanism involved in the uptake of long chain fatty acids. With isolated jejunal mucosal cells, the cellular uptake rate of deoxycholic acid or chenodeoxycholic acid increased linearly with time, showed no evidence of saturation, and was not decreased by the presence of a monospecific antibody to the membrane fatty acid binding protein. In contrast, oleate uptake was saturable, was inhibited by the same antibody, but was not affected by the presence of chenodeoxycholic acid or deoxycholic acid. Bile acid uptake by isolated enterocytes occurred at one-eighth the rate of fatty acid uptake if expressed in relation to total solute concentration; if expressed in relation to monomeric concentration, initial bile acid uptake was four orders of magnitude slower than fatty acid uptake. In the isolated perfused jejunal segment, chenodeoxycholic acid and deoxycholic acid uptake was not influenced by the presence of the antibody to membrane fatty acid binding protein, whereas absorption of oleate was inhibited by more than 70%. These experiments indicate that absorption of unconjugated lipophilic dihydroxy bile acids in the rodent jejunum does not involve the carrier mediated uptake mechanism involved in the absorption of long chain fatty acids—the mechanism is likely to be passive nonionic diffusion. the American Oil Chemists' Society (AOCS), 1990 Stremmel Wolfgang Division of Gastroenterology, Department of Internal Medicine, University Clinics of Dusseldorf, Moorenstrasse 5, 4000, Dusseldorf, Federal Republic of Germany aut Hofmann Alan Division of Gastroenterology, Department of Medicine, School of Medicine, University of California at San Diego, 92093, La Jolla, CA aut Lipids Springer-Verlag 25/1(1990-01-01), 11-16 0024-4201 25:1<11 1990 25 11745 https://doi.org/10.1007/BF02562421 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02562421 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stremmel Wolfgang Division of Gastroenterology, Department of Internal Medicine, University Clinics of Dusseldorf, Moorenstrasse 5, 4000, Dusseldorf, Federal Republic of Germany aut NATIONALLICENCE 700 1- Hofmann Alan Division of Gastroenterology, Department of Medicine, School of Medicine, University of California at San Diego, 92093, La Jolla, CA aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/1(1990-01-01), 11-16 0024-4201 25:1<11 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer