caa a22 4500 465765408 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02562430 doi (NATIONALLICENCE)springer-10.1007/BF02562430 Binding of galactosylsphingosine (psychosine) by albumin [Elektronische Daten] [Hideki Igisu, Masato Matsuoka, Naotaka Hamasaki] On applying [3H]galactosylsphingosine (psychosine) and albumin to a gel filtration column, either as a pre-incubated mixture or as separate solutions, the lipid and albumin co-eluted. When albumin and [3H]galactosylsphingosine were incubated with Sephadex G-50, the concentration of galactosylsphingosine outside the gel increased as the concentration of albumin increased. These observations indicate that albumin binds galactosylsphingosine. By using an equilibrium gel procedure, it was estimated that one molecule of albumin has 7.4 binding sites for galactosylsphingosine, and that the dissociation constant is 3.9×10−6 M. These values appear compatible with the potency of albumin to suppress galactosylsphingosine-induced hemolysis. the American Oil Chemists' Society (AOCS), 1990 Igisu Hideki Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Yahata-Nishi-Ku, 807, Kitakyushu, Japan aut Matsuoka Masato Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Yahata-Nishi-Ku, 807, Kitakyushu, Japan aut Hamasaki Naotaka Department of Clinical Chemistry and Laboratory Medicine, Faculty of Medicine, Fukuoka University, 814-01, Fukuoka, Japan aut Lipids Springer-Verlag 25/1(1990-01-01), 65-68 0024-4201 25:1<65 1990 25 11745 https://doi.org/10.1007/BF02562430 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02562430 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Igisu Hideki Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Yahata-Nishi-Ku, 807, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Matsuoka Masato Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Yahata-Nishi-Ku, 807, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Hamasaki Naotaka Department of Clinical Chemistry and Laboratory Medicine, Faculty of Medicine, Fukuoka University, 814-01, Fukuoka, Japan aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/1(1990-01-01), 65-68 0024-4201 25:1<65 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer