caa a22 4500 465765459 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02562424 doi (NATIONALLICENCE)springer-10.1007/BF02562424 Effects of dietary fish oil on biliary phospholipids and prostaglandin synthesis in the cholesterol-fed pairie dog [Elektronische Daten] [Michael Booker, Thayer Scott, Wayne La Morte] Cholesterol gallstone formation in the prairie dog is accompanied by an increase in the percentage of biliaryphospholipids containing arachidonic acid, and an increase in gallbladder prostaglandin (PG) synthesis, but the pathogenetic significance of these changes is unclear. Dietary supplementation with eicosapentaenoic acid (EPA), an omega-3 fatty acid which is commonly found in fish oil, decreases prostaglandin synthesis in some tissues by replacing arachidonic acid, and by competitively inhibiting prostaglandin synthesis. We studied the effect of dietary fish oil on gallbladder PG synthesis, and the relative abundance of various molecular species of phosphatidylcholines and phosphatidylethanolamines in bile and gallbladder epithelium in the cholesterol-fed prairie dog. Prairie dogs were maintained for 4 weeks on one of four diets: i) control, ii) cholesterol-supplemented (0.34%), iii) menhaden oil (50 g/kg chow), or iv) cholesterol plus menhaden oil. Supplementation with menhaden oil resulted in a replacement of arachidonic and linoleic acids with EPA and docosahexaenoic acids in the phospholipids of bile and gallbladder mucosa. In cholesterol-fed animals, supplementation with menhaden oil prevented increased gallbladder PG synthesis. Menhaden oil also reduced the incidence of cholesterol monohydrate crystals among cholesterol-fed animals (9/20 with cholesterol plus menhaden oil vs 21/22 with cholesterol alone), but the improvement could not clearly be attributed to decreased PG synthesis since supplementation with menhaden oil also increased the total phospholipid concentration in bile, and decreased the degree of cholesterol saturation. These results demonstrate that dietary supplementation with omega-3 fatty acids significantly influences biliary phospholipids, and decreases the incidence of cholesterol monohydrate crystal formation in this animal model. the American Oil Chemists' Society (AOCS), 1990 Booker Michael Surgical Research Section, Department of Surgery, Boston University Medical School, 8O East Concord St., O2118, Boston, MA aut Scott Thayer Surgical Research Section, Department of Surgery, Boston University Medical School, 8O East Concord St., O2118, Boston, MA aut La Morte Wayne Surgical Research Section, Department of Surgery, Boston University Medical School, 8O East Concord St., O2118, Boston, MA aut Lipids Springer-Verlag 25/1(1990-01-01), 27-32 0024-4201 25:1<27 1990 25 11745 https://doi.org/10.1007/BF02562424 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02562424 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Booker Michael Surgical Research Section, Department of Surgery, Boston University Medical School, 8O East Concord St., O2118, Boston, MA aut NATIONALLICENCE 700 1- Scott Thayer Surgical Research Section, Department of Surgery, Boston University Medical School, 8O East Concord St., O2118, Boston, MA aut NATIONALLICENCE 700 1- La Morte Wayne Surgical Research Section, Department of Surgery, Boston University Medical School, 8O East Concord St., O2118, Boston, MA aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/1(1990-01-01), 27-32 0024-4201 25:1<27 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer