caa a22 4500 465765467 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02562422 doi (NATIONALLICENCE)springer-10.1007/BF02562422 The uptake of (R,R,R)α-tocopherol by human endothelial cells in culture [Elektronische Daten] [Alvin Chan, Khai Tran] Endothelial cells from human umbilical cord vein in culture incorporate physiological and pharmacological amounts of (R,R,R)α-tocopherol in a time-dependent and dose-dependent manner. Incorporated tocopherol was found to associate predominantly with membrane fractions of the cell. When expressed on the basis of organelle protein, the highest amount of tocopherol was found in plasma membrane, and decreasing amounts in mitochondria, endoplasmic reticulum and cytosol. With the relatively wide range of tocopherol concentrations used in these studies (23.2-92.8 μM), there was no apparent toxicity on the cells as judged by unaltered cell numbers and cell viability. When the cells were enriched with tocopherol and cell tocopherol levels were monitored in tocopherol-free medium, there was a rapid phase of tocopherol disappearance, which was followed by a slower phase. The half-time for the disappearance of incorporated tocopherol was found to be approximately 65±8.6 hr (mean±SD, n=3). The results of this study clearly show that human endothelial cells in culture are a feasible model for the study of vitamin E uptake. The cell culture model could potentially be used to study other fat-soluble vitamins and essential nutrients. the American Oil Chemists' Society (AOCS), 1990 Chan Alvin Department of Biochemistry, Faculty of Health Sciences, University of Ottawa, 451 Smyth Rd., K1H 8M5, Ottawa, Ontario, Canada aut Tran Khai Department of Biochemistry, Faculty of Health Sciences, University of Ottawa, 451 Smyth Rd., K1H 8M5, Ottawa, Ontario, Canada aut Lipids Springer-Verlag 25/1(1990-01-01), 17-21 0024-4201 25:1<17 1990 25 11745 https://doi.org/10.1007/BF02562422 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02562422 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chan Alvin Department of Biochemistry, Faculty of Health Sciences, University of Ottawa, 451 Smyth Rd., K1H 8M5, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Tran Khai Department of Biochemistry, Faculty of Health Sciences, University of Ottawa, 451 Smyth Rd., K1H 8M5, Ottawa, Ontario, Canada aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/1(1990-01-01), 17-21 0024-4201 25:1<17 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer