caa a22 4500 465765491 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02544345 doi (NATIONALLICENCE)springer-10.1007/BF02544345 Purification of plasmalogens using Rhizopus delemar lipase and Naja naja naja phospholipase A2 [Elektronische Daten] [Yutaka Hirashima, Akhlaq Farooqui, Eric Murphy, Lloyd Horrocks] Bovine heart ChoGpl (choline glycerophospholipid) and bovine brain EtnGpl (ethanolamine glycerophospholipid) contain diacyl, alkenylacyl and alkylacyl analogs. Purification of plasmalogens was achieved usingR. delemar lipase andN. naja naja phospholipase A2 digestion. TheR. delemar lipase hydrolyzes the acyl bond at the 1-position of 1,2-diacyl glycerophospholipids. TheN. naja naja phospholipase A2 has greater activity with diacyl and alkylacyl than with alkenylacyl glycerophospholipids. These enzymes were mainly used to remove diacyl and alkylacyl analogs respectively. When the diacyl types were removed by double incubation withR. delemar lipase, the plasmalogen content was 94.2%±0.21% (mean±S.E.M., n=4) for PlsCho (plasmenylcholine) and 94.9%±0.19% (mean±S.E.M., n=3) for PlsEtn (plasmenylethanolamine). Recoveries were 74% and 88% respectively. These partially purified plasmalogens were treated withN. naja naja phospholipase A2. Finally, 97.7%±0.24% (mean±S.E.M., n=4) and 98.8%±0.27% (mean±S.E.M., n=3) pure plasmalogens were obtained for PlsCho and PlsEtn respectively. Plasmalogens were recovered in an overall yield of 7.7%±0.7% (mean±S.E.M., n=4) and 10.2%±1.2% (mean±S.E.M., n=3) for PlsCho and PlsEtn. American Oil Chemists' Society, 1990 Hirashima Yutaka Department of Physiological Chemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH aut Farooqui Akhlaq Department of Physiological Chemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH aut Murphy Eric Department of Physiological Chemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH aut Horrocks Lloyd Department of Physiological Chemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH aut Lipids Springer-Verlag 25/6(1990-06-01), 344-348 0024-4201 25:6<344 1990 25 11745 https://doi.org/10.1007/BF02544345 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544345 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirashima Yutaka Department of Physiological Chemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH aut NATIONALLICENCE 700 1- Farooqui Akhlaq Department of Physiological Chemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH aut NATIONALLICENCE 700 1- Murphy Eric Department of Physiological Chemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH aut NATIONALLICENCE 700 1- Horrocks Lloyd Department of Physiological Chemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/6(1990-06-01), 344-348 0024-4201 25:6<344 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer