caa a22 4500 465765521 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02544340 doi (NATIONALLICENCE)springer-10.1007/BF02544340 Effect of dietary fat on the lipid composition and utilization of short-chain fatty acids by rat colonocytes [Elektronische Daten] [Atif Awad, Sandra Ferger, Carol Fink] The objective of the present studies was to examine the effect of dietary fat on the lipid composition of rat colonocytes and their utilization of short-chain fatty acids (SCFA). Rats were fed 14% beef fat, fish oil or safflower oil plus 2% corn oil in a semi-synthetic base diet for 4 wk. Colonocytes were isolated and their lipid composition was examined. Feeding beef fat and fish oil resulted in an increase in monounsaturated fatty acids and a reduction in ω-6 fatty acids. Feeding fish oil resulted in an enrichment with ω-3 fatty acids. These was no dietary influence on the amount of either cholesterol or phospholipids of colonocytes. Fish oil feeding resulted in significant increase in colonocyte free fatty acids (FFA) as compared to other diets. Dietary fat was found to have no effect on SCFA utilization by colonocytes. Colonocytes were found to utilize SCFA in the order of butyrate ≥acetate ≥propionate. The presence of acetate and propionate in the medium had no effect on the rate of butyrate utilization. American Oil Chemists' Society, 1990 Awad Atif Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, 14214, Buffalo, NY aut Ferger Sandra Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, 14214, Buffalo, NY aut Fink Carol Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, 14214, Buffalo, NY aut Lipids Springer-Verlag 25/6(1990-06-01), 316-320 0024-4201 25:6<316 1990 25 11745 https://doi.org/10.1007/BF02544340 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544340 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Awad Atif Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, 14214, Buffalo, NY aut NATIONALLICENCE 700 1- Ferger Sandra Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, 14214, Buffalo, NY aut NATIONALLICENCE 700 1- Fink Carol Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, 14214, Buffalo, NY aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/6(1990-06-01), 316-320 0024-4201 25:6<316 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer