caa a22 4500 46576553X CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02544339 doi (NATIONALLICENCE)springer-10.1007/BF02544339 Modification of phospholipid polar head group with monomethylethanolamine and dimethylethanolamine decreases cholesteryl ester and triacylglycerol synthesis in cultured human fibroblasts [Elektronische Daten] [Cecile Maziere, Martine Auclair, Liliana Mora, Jean-Claude Maziere] Modification of the phospholipid polar head group was achieved by supplementation of the growth medium of cultured human fibroblasts with the choline analogues monomethylethanolamine (ME) or dimethylethanolamine (DE) at a concentration of 80-200 μg/mL for 48 hr. The maximum concentration of phosphatidylmonomethylethanolamine (PME) or phosphatidyldimethylethanolemine (PDE) reached without affecting the phospholipid/protein ratio was about 45% of total phospholipids. Incorporation of oleic acid into cholesteryl esters and triacylglycerols was markedly inhibited after supplementation with ME or DE, and accounted for 60% and 40% of controls, respectively, at 200 μg/mL, whereas incorporation into phospholipids was not affected. AcylCoA:cholesterol acyltransferase (ACAT) and diacylglycerol acyltransferase (DGAT) activities measured on cell-free extracts appeared to be decreased also by phospholipid polar head group modification, whereas the overall phospholipid acyltransferase activity remained unchanged. The intracellular content of cholesteryl esters and triacylglycerols, determined by the isotopic equilibrium method with radioactive cholesterol and glycerol, was found to be diminished to 50-60% and 40-50% of controls, respectively, after supplementation with the choline analogues. The study showed that modification of the phospholipid polar head group affects the activity of membrane-bound enzymes involved in the metabolism of neutral lipids. American Oil Chemists' Society, 1990 Maziere Cecile Faculte de Medecine Saint-Antoine, 27 rue Chaligny, 75012, Paris, France aut Auclair Martine Faculte de Medecine Saint-Antoine, 27 rue Chaligny, 75012, Paris, France aut Mora Liliana Faculte de Medecine Saint-Antoine, 27 rue Chaligny, 75012, Paris, France aut Maziere Jean-Claude Faculte de Medecine Saint-Antoine, 27 rue Chaligny, 75012, Paris, France aut Lipids Springer-Verlag 25/6(1990-06-01), 311-315 0024-4201 25:6<311 1990 25 11745 https://doi.org/10.1007/BF02544339 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544339 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maziere Cecile Faculte de Medecine Saint-Antoine, 27 rue Chaligny, 75012, Paris, France aut NATIONALLICENCE 700 1- Auclair Martine Faculte de Medecine Saint-Antoine, 27 rue Chaligny, 75012, Paris, France aut NATIONALLICENCE 700 1- Mora Liliana Faculte de Medecine Saint-Antoine, 27 rue Chaligny, 75012, Paris, France aut NATIONALLICENCE 700 1- Maziere Jean-Claude Faculte de Medecine Saint-Antoine, 27 rue Chaligny, 75012, Paris, France aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/6(1990-06-01), 311-315 0024-4201 25:6<311 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer