caa a22 4500 465765564 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02544337 doi (NATIONALLICENCE)springer-10.1007/BF02544337 Phospholipase a activity of cultured rat ventricular myocyte is affected by the nature of cellular polyunsaturated fatty acids [Elektronische Daten] [Gilles Nalbone, Alain Grynberg, Annick Chevalier, Jeannie Leonardi, Elise Termine, Huguette Lafont] Fatty acid composition of membrane phospholipids of cultured cardiomycotes can be modified by the type of polyunsaturated fatty acids (n−3 or n−6 PUFA) constituting the culture medium. In this study, we investigated the effect of fatty acid modification on the activities of the key enzymes involved in the deacylation-reacylation cycle of membrane phospholipids. Results showed that cardiomyocytes grown in the presence of n−6 PUFA exhibited a higher specific alkaline phospholipase A (mainly A2) activity (+34%) and a moderately lower lysophospholipase activity(−17%) than when incubated with n−3 PUFA. AcylCoA:lysophosphatidylcholine acyltransferase, acid lysosomal phospholipase A1 and acylCoA synthetase activities were not significantly altered by changes in cellular PUFA composition. It was demonstrated that the differences between phospholipase A activities of the two types of cultured cells were linked neither to a differential leakage of enzyme nor to oxidative injury to the enzyme through blockage of essential sulfhydryl groups. One likely explanation is that the PUFA-induced changes in membrane composition alter membrane physical properties which, in turn, affect membrane-bound phospholipase A activity. Possible beneficial effects of the n−3 PUFA-induced changes on membrane stability are discussed. American Oil Chemists' Society, 1990 Nalbone Gilles Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France aut Grynberg Alain Institute National de la Recherche Agronomique, 17 rue Sully, 21034, Dijon, France aut Chevalier Annick Institute National de la Recherche Agronomique, 17 rue Sully, 21034, Dijon, France aut Leonardi Jeannie Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France aut Termine Elise Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France aut Lafont Huguette Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France aut Lipids Springer-Verlag 25/6(1990-06-01), 301-306 0024-4201 25:6<301 1990 25 11745 https://doi.org/10.1007/BF02544337 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544337 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nalbone Gilles Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France aut NATIONALLICENCE 700 1- Grynberg Alain Institute National de la Recherche Agronomique, 17 rue Sully, 21034, Dijon, France aut NATIONALLICENCE 700 1- Chevalier Annick Institute National de la Recherche Agronomique, 17 rue Sully, 21034, Dijon, France aut NATIONALLICENCE 700 1- Leonardi Jeannie Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France aut NATIONALLICENCE 700 1- Termine Elise Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France aut NATIONALLICENCE 700 1- Lafont Huguette Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/6(1990-06-01), 301-306 0024-4201 25:6<301 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer