caa a22 4500 465765572 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02544342 doi (NATIONALLICENCE)springer-10.1007/BF02544342 Identification of carrot inositol phospholipids by fats atom bombardment mass spectrometry [Elektronische Daten] [Richard van Breemen, Jeffery Wheeler, Wendy Boss] Inositol phospholipids from carrot cell membranes grown in suspension cultured were purified by thinlayer chromatography (TLC) or column chromatography and tentatively identified by co-migration on TLC with animal inositol phospholipid standards. For more rigorous chemical characterization, carrot inositol phospholipids were then analyzed by negative ion fast atom bombardment mass spectrometry (FABMS). One phosphatidylinositol (PI), two lysophosphatidylinositols (LPI), and one phosphatidylinositol monophosphate (PIP) were identified in the carrot samples by the observation of ions [M-H]− and numerous fragment ions in the negative FAB mass spectra. MS/MS analysis were carried out to obtain further structural information of these phospholipids using a double-focusing mass spectrometer in which the magnetic sector (B) and the electrostatic analyzer (E) were scanned at a constant ratio (B/E). These B/E linked scans provided fragment ions of selected precursor ions while eliminating matrix and other contaminating ions. No molecular ions were detected for lysophosphatidylinositol monophosphate (LPIP) or phosphatidylinositol bisphosphate (PIP2), but fragment ions corresponding to these structures were observed. The primary fatty acids present in the carrot inositol phospholipids were linoleic (18∶2) and palmitic (16∶0) acids, whereas animal lipids contained arachidonic (20∶4), stearic (18∶0), linoleic, and palmitic acids. The only phosphatidylinositol found in carrot cells was palmitoyl linoleoyl PI. American Oil Chemists' Society, 1990 van Breemen Richard Department of Chemistry, North Carolina State University, Box 8204, 27695, Raleigh, NC aut Wheeler Jeffery Department of Botany, North Carolina State University, 27695, Raleigh, NC aut Boss Wendy Department of Botany, North Carolina State University, 27695, Raleigh, NC aut Lipids Springer-Verlag 25/6(1990-06-01), 328-334 0024-4201 25:6<328 1990 25 11745 https://doi.org/10.1007/BF02544342 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544342 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Breemen Richard Department of Chemistry, North Carolina State University, Box 8204, 27695, Raleigh, NC aut NATIONALLICENCE 700 1- Wheeler Jeffery Department of Botany, North Carolina State University, 27695, Raleigh, NC aut NATIONALLICENCE 700 1- Boss Wendy Department of Botany, North Carolina State University, 27695, Raleigh, NC aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/6(1990-06-01), 328-334 0024-4201 25:6<328 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer