caa a22 4500 465765580 CHVBK 20180323111912.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF02544344 doi (NATIONALLICENCE)springer-10.1007/BF02544344 LCAT inhibitors interfere with the enzymatic determination of cholesterol and triglycerides [Elektronische Daten] [William Harris, Alan Rayford] 5,5-Dithiobis-(2-nitrobenzoic acid) (DTNB) andp-chloromercuriphenylsulfonic acid (PCMPS) are well-known sulfhydryl inhibitors that are used to inhibit lecithin: cholesterol acyltransferase (LCAT). They were each found to interfere with the enzymatic assays of cholesterol and triglycerides. DTNB falsely reduced the measured plasma cholesterol content, and falsely increased triglyceride readings. The inteference with the triglyceride assay could be largely prevented by blanking for glycerol. PCMPS had only a slight effect on the cholesterol assay, but falsely lowered the triglyceride readings to a great extent, even with glycerol-blanking. Thus, these inhibitors should be avoided when plasma samples are to be enzymatically analyzed for cholesterol or triglycerides. American Oil Chemists' Society, 1990 Harris William Lipid and Arteriosclerosis Prevention Clinic, Division of Clinical Pharmacology, Department of Medicine, University of Kansas Medical Center, 66103, Kansas City, KS aut Rayford Alan Lipid and Arteriosclerosis Prevention Clinic, Division of Clinical Pharmacology, Department of Medicine, University of Kansas Medical Center, 66103, Kansas City, KS aut Lipids Springer-Verlag 25/6(1990-06-01), 341-343 0024-4201 25:6<341 1990 25 11745 https://doi.org/10.1007/BF02544344 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02544344 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harris William Lipid and Arteriosclerosis Prevention Clinic, Division of Clinical Pharmacology, Department of Medicine, University of Kansas Medical Center, 66103, Kansas City, KS aut NATIONALLICENCE 700 1- Rayford Alan Lipid and Arteriosclerosis Prevention Clinic, Division of Clinical Pharmacology, Department of Medicine, University of Kansas Medical Center, 66103, Kansas City, KS aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/6(1990-06-01), 341-343 0024-4201 25:6<341 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer