caa a22 4500 465765610 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02537160 doi (NATIONALLICENCE)springer-10.1007/BF02537160 The differential effect of eicosapentaenoic acid and oleic acid on lipid synthesis and VLDL secretion in rabbit hepatocytes [Elektronische Daten] [Kent Benner, Anna Sasaki, Dale Gowen, Alice Weaver, William Connor] The suppression of plasma very low density lipoprotein (VLDL) triglyceride levels by dietary fish oils rich in polyunsaturated n−3 fatty acids has been attributed to decreased hepatic VLDL secretion. To investigate the effect of n−3 fatty acids on lipid metabolism and VLDL secretion in a tissue culture system, we incubated rabbit hepatocytes with oleic acid and eicosapentaenoic acid (EPA) and examined [3H]glycerol and [14C]fatty acid incorporation into hepatocyte triglyceride and phospholipid and into media VLDL. Glycerol incorporation studies showed that EPA failed to stimulate VLDL triglyceride secretion from hepatocytes as occurred with oleic acid (P<0.05). Oleic acid preferentially enhanced hepatocyte triglyceride synthesis while EPA stimulated significantly phospholipid synthesis (P<0.01). Varying the relative concentrations of oleic acid and EPA at a constant total fatty acid concentration corroborated preferential triglyceride synthesis from oleic acid. Synthesis shifted predominantly to phospholipids with increasing concentrations of EPA and lower levels of oleic acid. Incorporation of the [14C]fatty acids (800 μM) followed similar patterns: 87% of [14C]oleic acid was incorporated into hepatocyte triglyceride and 44% of [14C]EPA was assimilated in hepatocyte phospholipid (p<0.001). Fatty acids at trace concentrations (53 nM) showed a more divergent pattern of lipid incorporation: 60% of [14C]oleic acid was incorporated into triglyceride while 91% of [14CEPA was incorporated into phospholipid (p<0.001). We conclude that in primary rabbit hepatocyte culture, which appears to be a useful model to study lipid metabolism and VLDL secretion, EPA is avidly incorporated into phospholipid while oleic acid predominantly becomes esterified in triglyceride. In addition, EPA, unlike oleic acid, fails to stimulate hepatocyte VLDL secretion. These divergent effects on hepatocyte lipid metabolism are, at least in part, likely to be responsible for fish oil induced suppression of plasma triglycerides. American Oil Chemists' Society, 1990 Benner Kent Division of Gastroenterology, Oregon Health Sciences University, L-461, 3181 SW Sam Jackson Park Road, 97201, Portland, OR aut Sasaki Anna Section of Clinical Nutrition and Lipid Metabolism, Department of Medicine, Oregon Health Sciences University, 97201, Portland, Oregon aut Gowen Dale Section of Clinical Nutrition and Lipid Metabolism, Department of Medicine, Oregon Health Sciences University, 97201, Portland, Oregon aut Weaver Alice Section of Clinical Nutrition and Lipid Metabolism, Department of Medicine, Oregon Health Sciences University, 97201, Portland, Oregon aut Connor William Section of Clinical Nutrition and Lipid Metabolism, Department of Medicine, Oregon Health Sciences University, 97201, Portland, Oregon aut Lipids Springer-Verlag 25/9(1990-09-01), 534-540 0024-4201 25:9<534 1990 25 11745 https://doi.org/10.1007/BF02537160 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537160 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Benner Kent Division of Gastroenterology, Oregon Health Sciences University, L-461, 3181 SW Sam Jackson Park Road, 97201, Portland, OR aut NATIONALLICENCE 700 1- Sasaki Anna Section of Clinical Nutrition and Lipid Metabolism, Department of Medicine, Oregon Health Sciences University, 97201, Portland, Oregon aut NATIONALLICENCE 700 1- Gowen Dale Section of Clinical Nutrition and Lipid Metabolism, Department of Medicine, Oregon Health Sciences University, 97201, Portland, Oregon aut NATIONALLICENCE 700 1- Weaver Alice Section of Clinical Nutrition and Lipid Metabolism, Department of Medicine, Oregon Health Sciences University, 97201, Portland, Oregon aut NATIONALLICENCE 700 1- Connor William Section of Clinical Nutrition and Lipid Metabolism, Department of Medicine, Oregon Health Sciences University, 97201, Portland, Oregon aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/9(1990-09-01), 534-540 0024-4201 25:9<534 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer