caa a22 4500 465765645 CHVBK 20180323111913.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02537157 doi (NATIONALLICENCE)springer-10.1007/BF02537157 Tissue phospholipid fatty acid composition in genetically lean (Fa/−) or obese (fa/fa) zucker female rats on the same diet [Elektronische Daten] [Ph. Guesnet, J. Bourre, M. Guerre-Millo, G. Pascal, G. Durand] The fatty acid composition of serum total lipids, of phospholipids of various organs (liver, heart, kidney), and of nervous structures (brain, retina, sciatic nerve, myelin, synaptosomes) have been compared in lean (Fa/−) and genetically obese (fa/fa) Zucker female rats. Both received a standard commercial diet including 37% of 18∶2n−6 and 5% of n−3 polyunsaturated fatty acids (PUFA), 1.7% of which were in the form of 20∶5n−3 and 22∶6n−3. In comparison with lean rats, the results for the obese rats pointed out (i) no difference in the fatty acid composition of nervous structures: (ii) a decrease of 18∶2n−6 (from −8% to −35%) and of 20∶4n−6 (from −9% to −49%) in serum, liver and in kidney; this was compensated for by an increase in 20∶3n−6 (from +30% to +320%) and in total n−3 PUFA (from +68% to +76%); (iii) a decrease of 20∶4n−6 (−18%) and of 22∶6n−3 (−24%) in heart compensated for by an increase in 18∶2n−6 (+39%) and in 20∶3n−6 (+233%); and (iv) constant levels of total PUFA (n−6 and n−3) in the various fractions studied, except in serum where this level decreased (−23%). Finally, except for the nervous structures, tissue phospholipids of obese rats included a lower proportion of 20∶4n−6 and a higher proportion of 20∶3n−6. This resulted in a significant reduction in the 20∶4n−6/20∶3n−6 ratio; by contrast, the 20∶3n−6/18∶2n−6 ratio increased. The results suggest that in Zucker rats, the obese character (fa/fa) affects the desaturation-elongation process of 18∶2n−6 to 20∶4n−6 by specifically decreasing Δ5-desaturase activity. American Oil Chemists' Society, 1990 Guesnet Ph Station de Recherches de Nutrition, 78350, Jouy-en-Josas, France aut Bourre J. INSERM U26, Hopital Femand-Widal, 200 rue du Faubourg St. Denis, 75475, Paris, France aut Guerre-Millo M. INSERM U177, Institut des Cordeliers, 15 rue de l'Ecole de Medecine, 75270, Paris, France aut Pascal G. Laboratorie des Sciences de la Consommation INRA-CRJ, 78350, Jouy-en-Josas, France aut Durand G. Station de Recherches de Nutrition, 78350, Jouy-en-Josas, France aut Lipids Springer-Verlag 25/9(1990-09-01), 517-522 0024-4201 25:9<517 1990 25 11745 https://doi.org/10.1007/BF02537157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02537157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guesnet Ph Station de Recherches de Nutrition, 78350, Jouy-en-Josas, France aut NATIONALLICENCE 700 1- Bourre J. INSERM U26, Hopital Femand-Widal, 200 rue du Faubourg St. Denis, 75475, Paris, France aut NATIONALLICENCE 700 1- Guerre-Millo M. INSERM U177, Institut des Cordeliers, 15 rue de l'Ecole de Medecine, 75270, Paris, France aut NATIONALLICENCE 700 1- Pascal G. Laboratorie des Sciences de la Consommation INRA-CRJ, 78350, Jouy-en-Josas, France aut NATIONALLICENCE 700 1- Durand G. Station de Recherches de Nutrition, 78350, Jouy-en-Josas, France aut NATIONALLICENCE 773 0- Lipids Springer-Verlag 25/9(1990-09-01), 517-522 0024-4201 25:9<517 1990 25 11745 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465765645 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer